Abstract
We report on the isolation and characterization of nine microsatellite markers in the takin (Budorcas taxicolor) from genomic DNA-enriched libraries. Twenty-eight microsatellites were screened from the libraries, and nine of the screened microsatellites were polymorphic. The number of observed alleles for each locus in 28 individuals ranged from two to seven, and the expected and observed heterozygosity was 0.105–0.758 and 0.071–0.821, respectively. Four loci (TK01, TK02, TK04 and TK08) of nine deviated from Hardy-Weinberg expectation and no significant linkage association was found among all these loci. These microsatellite markers provide useful tool for population genetic studies of the takin.
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Acknowledgements
This work was financial supported by projects of the National Natural Science Foundation of China (No. 30770304). We gratefully thank Mr. Gong Hui-Sheng, Mr. Gong Ming-Hao and China Adventure Travel (Zheng An) Ltd. for providing the takin samples.
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Zhang, Q., Zeng, ZG. & Song, YL. Isolation and characterization of nine microsatellite loci for the takin (Budorcas taxicolor). Conserv Genet 9, 1373–1375 (2008). https://doi.org/10.1007/s10592-007-9496-7
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DOI: https://doi.org/10.1007/s10592-007-9496-7