Abstract
The Australian freshwater cod genus, Maccullochella is represented by three species: Murray cod, M. peelii peelii, eastern freshwater cod, M. ikei, and trout cod, M.macquariensis. Seven novel microsatellite loci from M. ikei and six previously published loci from M. peelii peelii were tested on wild populations of Murray, eastern and trout cod. Levels of polymorphism varied between species with 13 loci polymorphic in Murray cod, 9 in trout cod and 7 in eastern cod. Observed heterozygosities ranged from 0.053 to 0.842. This suite of microsatellite loci will facilitate future studies of the genetic status of wild and hatchery bred populations of Maccullochella.
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Allendorf FW, Luikart G (2007) Conservation and the genetics of populations. Blackwell Publishing, Oxford
Brownstein MJ, Carpten JD, Smith JR (1996) Modulation of non-templatednucleotide addition by Taq DNA polymerase: primer modifications that facilitategenotyping. Biotechniques 20:1004–1010
Environment Protection and Biodiversity Conservation Act 1999. http://www.environment.gov.au/epbc/index.html
Elphinstone MS, Hinten GN, Anderson MJ, Nock CJ (2003) An inexpensive and high-throughput procedure to extract and purify total genomic DNA for population studies. Mol Ecol Notes 3:317–320
Fischer D, Bachmann K (1998) Microsatellite enrichment in organisms with large genomes (Allium cepai L.). Biotechniques 24:796–800
Loughnan SR, Baranski MD, Robinson NA, Jones PL, Burridge CP (2004) Microsatellite loci for studies of wild and hatchery Australian Murray cod Maccullochella peelii peelii (Percichthyidae). Mol Ecol Notes 4:382–384
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Rice WR (1989) Analysing tables of statistical tests. Evolution 43:223–225
Rowland SJ (1989) Aspects of the history and fishery of the Murray cod, Maccullochella peelii (Mitchell) (Percichthyidae). Proc Linn Soc New South Wales 111:201–213
Rowland SJ (1993) Maccullochella ikei, an endangered species of freshwater cod (Pisces: Percichthyidae) from the Clarence River System, NSW and M. peelii mariensis, a new subspecies from the Mary River System, Qld. Rec Aust Mus 45:121–145
Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and for biologist programmers. In: Krawetz S, Misener S, (eds) Bioinformatics methods and protocols. Humana Press, Totowa, pp 365–386
Rychlik W, Rhoads R (1989) A computer program for choosing optimal oligonucleotides for filter hybridisation, sequencing and in vitro amplification of DNA. Nucleic Acids Res 17:8543–8551
Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning: a laboratory manual. Cold Spring Harbour Laboratory Press, New York
Acknowledgements
This research was supported by an ARC-Linkage grant between SCU and NSW DPI (Fisheries). Thanks to Gavin Hinten and Dani Tikel for support with library creation and to Martin Elphinstone and Merv Shepherd for advice on primer design. Samples were provided by NSW and Qld DPI (Fisheries) with the assistance of Gavin Butler, Ian Wooden, Anthony Moore and Dani Tikel.
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Nock, C.J., Baverstock, P.R. Polymorphic microsatellite loci for species of Australian freshwater cod, Maccullochella . Conserv Genet 9, 1353–1356 (2008). https://doi.org/10.1007/s10592-007-9488-7
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DOI: https://doi.org/10.1007/s10592-007-9488-7