Abstract
This study reports the isolation and characterization of eight microsatellite markers for the study of Philippine tarsiers (Tarsius syrichta), small primates endemic to this Southeast Asian archipelago. The markers were used to screen 14 Tarsius syrichta for allelic diversity. This suite of highly polymorphic microsatellites provides the first chance to genetically study parentage and dispersal patterns in Philippine tarsiers.
References
Brandon-Jones D, Eudey AA, Geissmann T, Groves CP, Melnick DJ, Morales JC, Shekelle M, Stewart C-B (2004) Asian primate classification. Int J Primatol 25:97–164
Dagosto M, Gebo DL (1997) Preliminary study of the Philippine tarsier in Leyte. Asian Primates 6:4–8
Goodman M, Porter CA, Czelusniak J, Page SL, Schneider H, Shoshani J, Gunnell G, Groves CP (1998) Toward a phylogenetic classification of primates based on DNA evidence complemented by fossil evidence. Mol Phylogenet Evol 9:585–598
IUCN (2007) 2007 IUCN Red List of Threatened Species. http://www.iucnredlist.org. Downloaded on 16 November 2007
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood- based paternity inference in natural populations. Mol Ecol 7:639–655
Meireles CM, Czelusniak J, Page SL, Wildman DE, Goodman M (2003) Phylogenetic position of tarsiers within the order primates: evidence from y-globin DNA sequences. In: Wright PC, Simons EL, Gursky S (eds) Tarsiers: past, present, and future. Rutgers University Press, New Brunswick, pp 145–160
Merker S, Driller C, Perwitasari-Farajallah D, Zahner R, Zischler H (2007) Isolation and characterization of 12 microsatellite loci for population studies of Sulawesi tarsiers (Tarsius spp.). Mol Ecol Notes 7:1216–1218
Neri-Arboleda I, Stott P, Arboleda NP (2002) Home ranges, spatial movements and habitat associations of the Philippine tarsier (Tarsius syrichta) in Corella, Bohol. J Zool 257:387–402
Raymond M, Rousset F (1995) genepop (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Shekelle M (2003) Taxonomy and biogeography of Eastern tarsiers. Doctoral thesis, Washington University, St. Louis
Acknowledgements
We thank J. Brosius for providing muscle tissue of a Philippine tarsier and R. Zahner for his valuable help in the lab. This work was supported by the European Commission (project INPRIMAT, contract QLRT-2001-01325). A research grant from First Gen, Philippines, provided funds for field sampling and laboratory materials.
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Merker, S., Driller, C., Neri-Arboleda, I. et al. Polymorphic microsatellite markers for Philippine tarsiers (Tarsius syrichta). Conserv Genet 9, 1349–1351 (2008). https://doi.org/10.1007/s10592-007-9487-8
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DOI: https://doi.org/10.1007/s10592-007-9487-8