Skip to main content


Log in

Isolation and characterization of 18 microsatellite loci in the Egyptian vulture (Neophron percnopterus)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript


We developed 18 new microsatellite loci for the endangered Egyptian vulture (Neophron percnopterus). Microsatellite loci were screened for variation in two different populations belonging to separate subspecies: the nominal N. p. percnopterus and the Canarian N. p. majorensis. Mean expected heterosygosities were respectively 0.51 and 0.46, while the mean number of alleles per locus was 4.7 and 3.9. These new markers allow further genetic studies for the endangered Canarian Egyptian Vulture.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.


  • BirdLife International (2007) Species factsheet: Neophron percnopterus. Downloaded from

  • Donázar JA, Negro JJ, Palacios CJ, Gangoso L, Godoy JA, Ceballos O, Hiraldo F, Capote N (2002) Description of a new subspecies of the Egyptian vulture (Accipitridae: Neophron percnopterus) from the Canary Islands. J Raptor Res 36(1):17–23

    Google Scholar 

  • Donázar JA, Gangoso L, Forero MG, Juste J (2005) Presence, richness and extinction of birds of prey in the mediterranean and macaronesian islands. J Biogeogr 32:1701–1713

    Article  Google Scholar 

  • Glenn TC, Cary T, Dust M, Hauswaldt S, Prince K, Clifton R, Shute I (2000) Microsatellite isolation.

  • Hamilton MB, Pincus EL, Di Fiore A, Flesher RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. Biotechniques 27:500–507

    PubMed  CAS  Google Scholar 

  • Koenig R (2006) Vulture research soars as the scavengers’ numbers decline. Science 312:1591–1592

    Article  PubMed  CAS  Google Scholar 

  • Kretzman MB, Capote N, Gautschi B, Godoy JA, Donázar JA, Negro JJ (2003) Genetically distinct island population of the Egyptian vulture (Neophron percnopterus). Conserv Genet 4:697–706

    Article  Google Scholar 

  • Levy N (1996) Present status, distribution and conservation trends of the Egyptian vulture (Neophron percnopterus) in the Mediterranean countries and adjacent arid regions. In: J Muntaner, J Mayol (eds) Biology and Conservation of Mediterranean Raptors (Monographic 4). Sociedad Española de Ornitología, Palma de Mallorca, microsatellite data. Mol Ecol Notes, 4:535–538 (pp 13–33)

  • Rousset F, Raymond M (1995) Testing heterozygotes excess and deficiency. Genetic 140:1413–1419

    CAS  Google Scholar 

  • Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory press, New York

    Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

Download references


We thank Ana Trujillano, Laura Gangoso J. Manuel Grande, Olga Ceballos, Ainara Cortés and Manuel de la Riva for their fieldwork assistance. We also thank Inés Rico for her assistance in laboratory procedures and the genomic library development. We want to thank to Cabildo of Fuerteventura and INIPRO (LIFE04 NAT/ES/000067) for their logistic facilities. This work has been financed by the Ministry of Science and Education of Spain (Project CGL2004–00270).

Author information

Authors and Affiliations


Corresponding author

Correspondence to Rosa Agudo.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Agudo, R., Roques, S., Galarza, J.A. et al. Isolation and characterization of 18 microsatellite loci in the Egyptian vulture (Neophron percnopterus). Conserv Genet 9, 1345–1348 (2008).

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: