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Microsatellite markers in the endangered Australian northern corroboree frog, Pseudophryne pengilleyi (Anura: Myobatrachidae) and amplification in other Pseudophryne species

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Abstract

Seven microsatellite primer pairs were isolated and characterized in the endangered Australian northern corroboree frog (Pseudophryne pengilleyi). All seven were polymorphic (2–14 alleles) and displayed high heterozygosity (0.036–0.964) in 28 sampled individuals. We also tested the microsatellites on two closely related species. Four were polymorphic in the southern corroboree frog (P. corroboree) and Bibron’s toadlet (P. bibronii). These primers will be useful in studies of conservation genetics and mating systems in Pseudophryne species.

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Acknowledgements

This study was funded by grants from the Australian Academy of Science Endangered Species Fund (MJM) and the Australian Research Council (PJB & JSK). We thank Ian Scott for early help in the lab and Rod Pietsch, Dave Hunter, Will Osborne, Gerry Marantelli and The Amphibian Research Centre for access to tissues. This work was carried with Animal Care and Ethics Committee approval from the Australian National University (Protocol No. F.BTZ.64.04) and the NSW Department of Environment and Conservation (AEC No. 041025/03) and under scientific license from the NSW National Parks and Wildlife Service (licence No. S11125 to DH, and licence No. S11005 to PB).

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Authors and Affiliations

  1. School of Botany and Zoology, Australian National University, Canberra, ACT, 0200, Australia

    Matthew J. Morgan, Phillip G. Byrne, Christine M. Hayes & J. Scott Keogh

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  1. Matthew J. Morgan
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  2. Phillip G. Byrne
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  3. Christine M. Hayes
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  4. J. Scott Keogh
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Correspondence to J. Scott Keogh.

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Morgan, M.J., Byrne, P.G., Hayes, C.M. et al. Microsatellite markers in the endangered Australian northern corroboree frog, Pseudophryne pengilleyi (Anura: Myobatrachidae) and amplification in other Pseudophryne species. Conserv Genet 9, 1315–1317 (2008). https://doi.org/10.1007/s10592-007-9468-y

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  • DOI: https://doi.org/10.1007/s10592-007-9468-y

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