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Characterization of novel microsatellite loci in the great leaf-nosed bat, Hipposideros armiger and cross-amplification in other related species

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Abstract

Eight microsatellite loci were isolated from an enriched genomic library of the great leaf-nosed bat, Hipposideros armiger. The polymorphism of these loci was tested on a population of 48 individuals from Anhui Province, China. All loci revealed the polymorphism ranging from three to 12 alleles. The observed heterozygosity values were from 0.213 to 0.875 and expected heterozygosity from 0.232 to 0.820. No significant linkage disequilibrium was detected. Two loci significantly deviated from Hardy–Weinberg equilibrium after Bonferroni correction. In addition, successful cross-amplification also suggested that these microsatellite markers will facilitate research on the population genetics and gene flow of H. armiger and other related species.

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References

  • Bates PJJ, Harrison DL (1997) Bats of the Indian subcontinent. Harrison Zoological Museum Publications, Sevenoaks

    Google Scholar 

  • Bogdanowicz W, Fenton MB, Daleszczyk K (1999) The relationships between echolocation calls, morphology and diet in insectivorous bats. J Zool 247:381–393

    Article  Google Scholar 

  • Gu XM (2002) The karyotype analysis of Hipposideros armiger. Chinese J Zool 37:19–21

    Google Scholar 

  • Hua PY, Chen JP, Sun M, Liang B, Zhang SY, Wu DH (2006) Characterization of microsatellite loci in fulvous fruit bat Rousettus leschenaulti. Mol Ecol Notes 6:939–941

    Article  CAS  Google Scholar 

  • Karp A, Isaac PG, Ingram DS (1998) Molecular tools for screening biodiversity. Chapman & Hall, London

    Google Scholar 

  • Li G, Jones G, Rossiter SJ, Chen SF, Parsons S, Zhang SY (2006) Phylogenetics of small Horseshoe bats from east Asia based on mitochondrial DNA sequence variation. J Mammal 87:1234–1240

    Article  Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2)—population genetics software for exact tests and ecumenicism. J Hered 86: 248–249

    Google Scholar 

  • Sambrook J, Russell DW (2002) Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Clod Spring Harbor

    Google Scholar 

  • Simmons NB (2005) Order chiroptera. In: Wilson DE, Reeder DM (eds) Mammal species of the world: a taxonomic and geographic reference. The Johns Hopkins University Press, Baltimore

    Google Scholar 

  • Van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-checker: software for identifying and correcting genotyping errors in mirosatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Wu Y, Masashi H, Li YH (2004) Karyology of seven species bats from Sichuan, China. Acta Theriologica Sinica 24:30–35

    Google Scholar 

  • Zhao HH, Zhang SY, Z MX, Zhou J (2003) Correlations between call frequency and ear length in bats belonging to the families Rhinolophidae and Hipposideridae. J Zool 259:189–195

    Article  Google Scholar 

Download references

Acknowledgements

This work was supported by Zijiang scholarship to Shuyi Zhang, and by National Science Council of Taiwan, ROC to LK Lin (NSC96-2621-b-029-002). We thank Hui Zhong, Li Wei for assistance in the samples collection of H. armiger.

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Correspondence to L. K. Lin or S. Y. Zhang.

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Guo, T.T., Hua, P.Y., Lin, L.K. et al. Characterization of novel microsatellite loci in the great leaf-nosed bat, Hipposideros armiger and cross-amplification in other related species. Conserv Genet 9, 1063–1065 (2008). https://doi.org/10.1007/s10592-007-9455-3

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  • DOI: https://doi.org/10.1007/s10592-007-9455-3

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