Abstract
We describe 12 new polymorphic dinucleotide microsatellite loci and multiplex Polymerase Chain Reaction conditions from the loggerhead sea turtle Caretta caretta. Levels of polymorphism were assessed in 50 individuals from the nesting population of the Cape Verde Islands. Number of alleles ranged from 3 to 13 (average of 7.33) and the values of observed heterozygosities from 0.32 to 0.80 (average of 0.61). Cross-species amplification on three other marine turtles, Chelonia mydas, Eretmochelys imbricata and Dermochelys coriacea, revealed polymorphism and variability at eight, eleven and three loci, respectively.
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References
Bowen BW, Bass AL, Chow S-M, Bostrom M, Bjorndal KA, Bolten AB, Okuyama T, Bolker BM, Epperly S, Lacasella E, Shaver D, Dodd M, Hopkins-Murphy SR, Musick JA, Swingle M, Rankin-Baransky K, Teas W, Witzell WN, Dutton PH (2004) Natal homing in juvenile loggerhead turtles (Caretta caretta). Mol Ecol 13:3797–3808
Bowen BW, Bass AL, Soares L, Toonen J (2005) Conservation implications of complex population structure: lessons from the loggerhead turtle (Caretta caretta). Mol Ecol 14:2389–2402
Carreras C, Pon S, Maffucci F, Pascual M, Barceló A, Bentivegna F, Cardona L, Alegre F, SanFélix M, Fernández G, Aguilar A (2006) Genetic structuring of immature loggerhead sea turtles (Caretta caretta) in the Mediterranean Sea reflects water circulation patterns. Mar Biol 149(5):1269–1279
Crim JL, Spotila LD, Spotila JR, O’ Connor M, Reina R, Williams CJ, Paladinos FV (2002) The leatherback turtle, Dermochelys coriacea, exhibits both polyandry and polygyny. Mol Ecol 11:2097–2106
Encalada SE, Bjorndal KA, Bolten AB, Zurita JC, Schroeder B, Possardt E, Sears CJ, Bowen BW (1998) Population structure of loggerhead turtle (Caretta caretta) nesting colonies in the Atlantic and Mediterranean as inferred from mitochondrial DNA control region sequences. Marine Biology 130:567–575
Excoffier L, Laval G, Schneider S (2005) Arlequin (version 3.0): An integrated software package for population genetics data analysis. Evol Bioinform Online 1:47–50
FitzSimmons NN, Moritz C, Moore SS (1995) Conservation and dynamics of microsatellite loci over 300 million years of marine turtle evolution. Mol Biol Evol 12(3):432–440
Glenn TC, Schable M (2003) Microsatellite Isolation with Dynabeads 2003©. http://www.uga.edu/srel/DNA_Lab/protocols.htm
Hall TA (1999) BioEdit: a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT. Nucl. Acids Symp Ser 41:95–98
Jensen MP, Abreu-Grobois FA, Frydenberg J, Loeschcke V (2006) Microsatellites provide insight into contrasting mating patterns in arribada vs. non-arribada olive ridley sea turtle rookeries. Mol Ecol 15(9):2567–2575
Kaska Y (2000) Genetic structure of Mediterranean sea turtle populations. Turk J Zool 24:191–197
Laurent L, Casale P, Bradai MN, Godley BJ, Gerosa G, Broderick AC, Schroth W, Scierwater B, Levy AM, Freggi D, Abd El-Mawla EM, Hadoud DA, Gomati HE, Domingo M, Hadjichristophorou M, Kornaraky L, Demirayak F, Gautier CH (1998) Molecular resolution of marine turtle stock composition in fishery by-catch: a case study in the Mediterranean. Mol Ecol 7:1529–1542
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7:639–655
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Rozen S, Skaletsky HJ (2000) Primer 3 on the www for general users and for biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: Methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386
Shamblin BM, Faitcloth BC, Dodd M, Wood-Jones A, Castleberry SB, Carroll JP, Nairn CJ (2007) Tetranucleotide microsatellites from the loggerhead sea turtle (Caretta caretta). Mol Ecol Notes 7:784–787
Acknowledgements
We thank to Pascual Calabuig, Nuria Varo, Ana Liria, Pedro López, and Paula Sanz for their contributions on sampling and field collection. Miguel Alcaide and Carlos Carreras helped us during laboratory procedures and the genomic library development. We are very grateful to Instituto Canario de Ciencias Marinas, Estación Biológica de Doñana, Junta de Andalucía, and the Cape Verde government for helping with the field and laboratory equipment. CMA was supported by a PhD grant from the Canary Islands government.
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Monzón-Argüello, C., Muñoz, J., Marco, A. et al. Twelve new polymorphic microsatellite markers from the loggerhead sea turtle (Caretta caretta) and cross-species amplification on other marine turtle species. Conserv Genet 9, 1045–1049 (2008). https://doi.org/10.1007/s10592-007-9446-4
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DOI: https://doi.org/10.1007/s10592-007-9446-4