Abstract
The golden-crowned sifaka (Propithecus tattersalli) is one of the most critically endangered species of Propithecus endemic to a limited area in the Daraina region of north-eastern Madagascar. This species is endangered due to habitat loss and fragmentation, a consequence of deforestation. Twenty nuclear microsatellite loci were isolated from genomic DNA derived from a Propithecus tattersalli from the Daraina area in north-eastern Madagascar. Population genetic parameters were estimated on 20 individuals from two Daraina forest fragments to determine the potential utility of this marker suite for future studies on the golden-crowned sifaka.
References
Dieringer D, Schlötterer C (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Mol Ecol Notes 3:167–169 Available: http://i122server.vu-wien.ac.at/MSA/info.html/MSA_info.html, Accessed 2 June 2006
Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:85–486
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Updated from Goudet (1995) Available: http://www.unil.ch/izea/softwares/fstat.html, Accessed 28 March 2006
Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds) Molecular systematics, 2nd edn. Sinauer Associates, Inc., Sunderland, MA, pp 321–381
Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-species markers. Proc Nat Acad Sci USA 91:88–92
Longmire JL, Gee GF, Hardekoff CL, Mark GA (1992) Establishing paternity in whooping cranes (Grus americana) by DNA analysis. Auk 109:522–529
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7(5):639–655
Mittermeier RA, Konstant WR, Hawkins F, Louis EE, Langrand O, Ratsimbazafy J, Rasoloarison R, Ganzhorn JU, Rajaobelina S, Tattersall I, Meyers DM (2005) Lemurs of Madagascar 2nd edn. Conservation International, Washington, pp. 373–381
Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) “Tools for developing molecular markers.” interdisciplinary center for biotechnology research laboratory manual. The University of Florida Gainesville, Florida, pp 11–65
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms J Hered 86:248–249. Available: http://wbiomed.curtin.edu.au/genepop/, Accessed 18 September 2007
Sambrook JE, Fritsch F, Maniatis T (1989) Molecular cloning: a laboratory manual. 2nd edn. Cold Spring Harbor Press, New York
Simons EL (1988) A new species of Propithecus (Primates) from north-east Madagascar. Folia Primatol 50:148–151
Slate J, Marshall TC, Pemberton JM (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Mol Ecol 9(6):801–808. Available: http://helios.bto.ed.ac.uk/evolgen/cervus/cervus.html, Accessed 28 August 2006
Tattersall I (1986) Notes on the distribution and taxonomic status of some subspecies of Propithecus in Madagascar. Folia Primatologica 46:51–63
Vargas A, Jiménez I, Palomeres F, Palacios MJ (2002) Distribution, status, and conservation needs of the golden-crowned sifaka (Propithecus tattersalli). Biol Conserv 108:325–334
Van Oosterhaut C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538. Available: http://www.microchecker.hull.ac.uk/, Accessed 6 March 2006
Acknowledgments
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support. This research was also supported by grants from the Ahmanson Foundation. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. This project would not have been possible without the support of the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET), the Association Fanamby, the Association Nationale pour la Gestion des Aires Protégées (ANGAP) and the Ministère des Eaux et Forêt, Madagascar.
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Razafindrakoto, A., Quéméré, E., Shore, G.D. et al. Characterization of 20 microsatellites marker loci in the golden-crowned sifaka (Propithecus tattersalli) . Conserv Genet 9, 1027–1031 (2008). https://doi.org/10.1007/s10592-007-9440-x
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DOI: https://doi.org/10.1007/s10592-007-9440-x