Abstract
Pugionium (Brassicaceae) is a small Central-Asian endemic genus with four tentative species described before. All of them grow in the desert habitat and have been considered as important psammophytes for evolutionary studies and ecological restorations. However, the wild resources of these species have been declined because of overexploitation. In this study, 12 polymorphic microsatellite loci have been developed for P. dolabratum Maxim, and the performance of primer pairs amplifying these loci in related three species has also been tested. The number of alleles in 18 individuals from 13 geographically distant populations ranged from three to seven per locus. The observed and expected heterozygosities varied from 0.17 to 0.30 and from 0.32 to 0.48, respectively. We further performed cross-priming tests in the remaining three species of this genus, P. cornutum, P. calcaratum and P. cristatum. These newly recovered microsatellite loci will be useful for studying the population genetics and adaptive evolution of these endangered psammophytes in the desert.
This is a preview of subscription content, log in via an institution to check access.
References
Clarke KR, Gorley RN (2001) PRIMER v5: user manual/tutorial. PRIMER-E Ltd., Plymouth, p 91
Hauswaldt JS, Glenn TC (2003) Microsatellite DNA loci from the Diamondback terrapin (Malaclemys terrapin). Mol Ecol Notes 3:174–176
Hsu YC, Li SH, Lin YS, Severinghaus LL (2006) Microsatellite loci from Lanyu scops owl (Otus elegans botelensis) and their cross-species application in four species of strigidae. Conserv Genet 7:161–165
Illarionova ID (1999) Synopsis of the genus Pugionium Gaertn (Brassicaceae). Komarovia 1:35–37
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Richards CM, Reilley A, Touchell D, Antolin MF, Walters C (2004) Microsatellite primers for Texas wild rice (Zizania texana), and a preliminary test of the impact of cryogenic storage on allele frequency at these loci. Conserv Genet 5:853–859
Setsuko S, Ueno S, Tsumura Y, Tomaru N (2005) Development of microsatellite markers in Magnolia stellata (Magnoliaceae), a threatened Japanese tree. Conserv Genet 6:317–320
Wang JY, Zhai S, Hao LZ (2004) Current situations and trends as well as exploiting strategies about psammophyte Pugionium cornutum (L.) Gaertn research and utilization. Agric Res Arid Areas 22(4):232–235 (in Chinese)
Zhang XF (1995) Revision of Pugionium dolabratum Maxim. var. platypterum H. L. Yang. Acta Phytotax Sin 33(5):502 (in Chinese)
Zhang DY, Chen N, Yang YZ, Zhang Q, Liu JQ (2007) Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae). Conserv Genet 8. doi:10.1007/s10592-007-9344-9
Zhao YZ (1999) A taxonomic revision and floristic analysis of the genus Pugionium. J Inn Mongolia Univ 31:107–109 (in Chinese)
Zhou TY, Guo RL, Lan YZ, Lu NL, Guan KJ, An ZX (1987) Brassicaceae. In: Flora of China, vol 33. Science Press, China, pp 68–70 (in Chinese)
Zhou Z, Miwa M, Hogetsu T (1999) Analysis of genetic structure of a Suillus grevillei population in a larix keampferi stand by polymorphism of inter-simple sequence repeat (ISSR). New Phytol 144:55–63
Acknowledgments
We thank Prof. Jianquan Liu for supervising this work and careful revision of the manuscript. We also thank Dr. Yuxia Wu, Dr. Qian Zhang, Dr. Ailan Wang, and Dr. Yuan Li for their helps in the experiments. This research was supported by the Education Ministry (NCET-05-886) and the Science and Technology Innovation Team of Gansu Desert Control Research Institute.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Yu, Q., Zhang, D., Wan, D. et al. Development of microsatellite DNA loci for Pugionium dolabratum (Brassicaceae), an endangered psammophyte. Conserv Genet 9, 1019–1022 (2008). https://doi.org/10.1007/s10592-007-9438-4
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-007-9438-4