Abstract
The blue mussel Mytilus trossulus occurs in the Pacific and in the North Atlantic. We developed and characterized six microsatellite loci for Baltic M. trossulus. Seventeen microsatellite loci were screened, of which six were polymorphic. The number of alleles among 50 individuals ranged from 3 to 13 and the observed and expected heterozygosity were 0.09–0.46 and 0.34–0.86, respectively. The loci were also tested for cross amplification in M. edulis, in which four of the six microsatellite loci were successfully amplified.
This is a preview of subscription content, log in via an institution to check access.
References
Chapuis M-P, Estoup A (2007) Microsatellite null alleles and estimation of population differentiation. Mol Biol Evol 24:621–631
Glenn TC, Schable NA (2005) Isolating microsatellite DNA loci. Methods Enzymol 395:202–222
Hedgecock D, Li G, Hubert S, Bucklin K, Ribes V (2004) Widespread null alleles and poor cross-species amplification of microsatellite DNA loci cloned from the Pacific oyster, Crassostrea gigas. J Shellfish Res 23:379–385
Kautsky N, Evans S (1987) Role of biodeposition by Mytilus edulis in the circulation of matter and nutrients in a Baltic coastal ecosystem. Mar Ecol Prog Ser 38:201–212
McGoldrick DJ, Hedgecock D, English LJ, Baoprasertkul P, Ward RD (2000) The transmission of microsatellite alleles in Australian and North American stocks of the Pacific oyster (Crassostrea gigas): selection and null alleles. J Shellfish Res 19:779–788
Murray MG, Thompson WF (1980) Rapid isolation of high molecular weight plant DNA. Nucleic Acids Res 8:4321–4325
Park SDE (2001) Trypanotolerance in West African Cattle and the population genetic effects of selection. Dissertation, University of Dublin
Presa P, Pérez M, Diz AP (2002) Polymorphic microsatellite markers for blue mussels (Mytilus spp.). Conserv Genet 3:441–443
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecumenicism. J Hered 86:248–249
Reece KS, Ribeiro WL, Gaffney PM, Carnegie RB, Allen SK Jr (2004) Microsatellite marker development and analysis in the eastern oyster, Crossaster virginica: confirmation of null alleles and non-Mendelian segregating ratios. J Hered 95:255–361
Rigonos C, Cunningham W (2005) Local adaptation and species segregation in two mussel (Mytilus edulis x Mytilus trossulus) hybrid zones. Mol Ecol 14:381–400
Seed R (1992) Systematics, evolution and distribution of mussels belonging to the genus Mytilus: an overview. Am Malacol Bull 9:123–137
van Oosterhout C, Hutchinson WF, Mills DPM, Shipley P (2004) micro-checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538
Varvio S-L, Koehn RK, Väinölä R (1988) Evolutionary genetics of the Mytilus edulis complex in the North Atlantic region. Mar Biol 98:51–60
Acknowledgements
This project was funded by Helge Ax:on Johnsons stiftelse and C.F. Lundströms stiftelse.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Gardeström, J., Pereyra, R.T. & André, C. Characterization of six microsatellite loci in the Baltic blue mussel Mytilus trossulus and cross-species amplification in North Sea Mytilus edulis . Conserv Genet 9, 1003–1005 (2008). https://doi.org/10.1007/s10592-007-9432-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-007-9432-x