Abstract
The crowned sifaka (Propithecus deckeni coronatus) is found between the Mahavavy the Betsiboka Rivers in either Anjahamena or Antrema Classified Forest. The crowned sifaka is endangered due to the loss and fragmentation of its natural habitat by deforestation. Twenty polymorphic nuclear microsatellite loci were isolated from genomic DNA derived from a P. d. coronatus from Anjahamena, Madagascar. Population genetic parameters were estimated on 10 individuals sampled from Anjahamena, Madagascar, to determine marker utility and preliminary baseline values to study the species’ genetic diversity and phylogeography. In a cross-amplification test, each marker in the suite was informative in P. d. deckeni.
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Acknowledgements
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. This research was also supported by grants from the Ahmanson Foundation, which have provided the laboratory with three ABI automated DNA sequencers. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo’s Madagascar Biodiversity and Biogeography Project. This project would not have been possible without the support of the staffs from the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET), the Association Nationale pour la Gestion des Aires Protégées (ANGAP) and the Ministère des Eaux et Foret, Madagascar. We also extend our thanks to student intern Robert Everson for laboratory assistance in optimizing the primer conditions.
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Lei, R., McGuire, S.M., Shore, G.D. et al. Characterization of 20 microsatellites developed from Propithecus deckeni coronatus with cross-amplification in Propithecus deckeni deckeni . Conserv Genet 9, 999–1002 (2008). https://doi.org/10.1007/s10592-007-9431-y
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DOI: https://doi.org/10.1007/s10592-007-9431-y