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Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species

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Abstract

Chloroplast microsatellite (cpSSR) markers were developed for three ecologically and economically important tree species in the mangrove family, Rhizophoraceae: Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa. Noncoding regions of chloroplast DNA (cpDNA) from each species were separately amplified using universal chloroplast primers. Six, two, and three polymorphic cpSSR loci in B. gymnorrhiza, K. candel, and R. stylosa, respectively, were developed from amplified noncoding cpDNA regions. Characterization of 216, 156, and 253 individuals of B. gymnorrhiza, K. candel, and R. stylosa, respectively, collected from different natural mangrove populations (B. gymnorrhiza, 9; K. candel, 7; R. stylosa, 9) on Iriomote Island in Japan showed that these loci provide cpSSR markers with polymorphisms ranging from two to four alleles per locus and gene diversity between 0.027 and 0.480. These cpSSR markers will be useful for analyzing the maternal lineage distributions and population genetic structures of the three species. Several of these markers may also be useful in similar studies of other mangrove species.

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Acknowledgments

This work was supported by Grants-in-Aid from the Japan Society for the Promotion of Science (JSPS). We are grateful to Prof. Tokushiro Takaso of the University of Ryukyus for his assistance in collecting samples.

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Authors and Affiliations

  1. Graduate School of Agricultural and Life Sciences, The University of Tokyo, Yayoi 1-1-1, Bunkyo-ku, Tokyo, 113-8657, Japan

    Md. Sajedul Islam & Taizo Hogetsu

  2. Asian Natural Environmental Science Center, The University of Tokyo, Midori-cho 1-1-8, Nishitokyo-Shi, Tokyo, 188-0002, Japan

    Chunlan Lian & Qifang Geng

  3. Faculty of Agriculture, University of the Ryukyus, 1 Senbaru, Nishihara, Okinawa, 903-0213, Japan

    Norikazu Kameyama

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  1. Md. Sajedul Islam
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  2. Chunlan Lian
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  3. Qifang Geng
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  4. Norikazu Kameyama
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  5. Taizo Hogetsu
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Correspondence to Chunlan Lian.

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Islam, M.S., Lian, C., Geng, Q. et al. Chloroplast microsatellite markers for the mangrove tree species Bruguiera gymnorrhiza, Kandelia candel, and Rhizophora stylosa, and cross-amplification in other mangrove species. Conserv Genet 9, 989–993 (2008). https://doi.org/10.1007/s10592-007-9424-x

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  • DOI: https://doi.org/10.1007/s10592-007-9424-x

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