Abstract
Sixty-eight primer sets for microsatellite loci were developed from microsatellite motif enriched genomic libraries of pooled DNA from the polyploid green and white sturgeon (Acipenser medirostris and A. transmontanus). Four individuals from each species were screened for polymorphism at these loci. Forty-eight loci amplified in both species, and some exhibited species-specific amplification for white or green sturgeon (8 and 12 loci, respectively). The number of alleles per locus ranged from one to 12. At least 68% of the green and 65% of the white sturgeon loci we developed are polysomic.
References
Adams P, Grimes C, Hightower J et al (2006) Population status of North American green sturgeon Acipenser medirostris. Environ Biol Fishes, DOI 10.1007/s10641-006-9062-z
Duke S, Anders P, Ennis G et al (1999) Recovery plan for Kootenai River white sturgeon (Acipenser transmontanus). J Appl Ichthyol 15:157–163
Israel J, Cordes J, Blumberg M et al (2004) Geographic patterns of genetic differentiation among collections of green sturgeon. N Am J Fish Man 24:922–931
Jones K, Levine K, Banks J (2002) Characterization of 11 polymorphic tetranucleotide microsatellites for forensic applications in California elk (Cervus elaphus canadensis). Mol Ecol Notes 2(4):425–427
Ludwig A, Belfiore NM, Pitra et al (2001) Genome duplication events and functional reduction of ploidy levels in sturgeon (Acipenser, Huso and Scaphirhynchus). Genetics 158:1203–1215
McQuown EC, Sloss BL, Sheehan RJ, Rodzen J, Tranah G, May B (2000) Microsatellite analysis of genetic variation in sturgeon: new primer sequences for Scaphirynchus and Acipenser. Trans Am Fish Soc 129:1380–1388
Rodzen J and May B (2002) Inheritance of microsatellite loci in the white sturgeon Acipenser transmontanus. Genome 45:1064–1076
Rodzen J, Agresti J, Tranah G et al (1998) Agarose overlays allow simplified staining of polyacrylamide gels. Biotechniques 25:584
Rodzen J, Famula T, May B (2004a) Estimation of parentage and relatedness in the polyploid white sturgeon (Acipenser transmontanus) using a dominant marker approach for duplicated microsatellite loci. Aquaculture 232:165–182
Rodzen JA, May B, Anders P, and Ireland S (2004b) Initial microsatellite analysis of wild Kootenai River white sturgeon and subset brood stock groups used in a conservation aquaculture program. US Department of Energy Technical Report Contract #88-64, Bonneville Power Administration
Sambrook J, Fritsch E, Maniatis T (1989) Molecular cloning, a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York
US Fish and Wildlife Service (1994) Endangered and threatened wildlife and plants: determination of endangered status for the Kootenai River white sturgeon population. Fed Regist 59(171):45989–46002
US Fish and Wildlife Service (1999) Recovery plan for the Kootenai River population of the white sturgeon (Acipenser transmontanus). Region 1 US Fish and Wildlife Service, Portland
US National Marine Fisheries Service (2006) Endangered and threatened wildlife and plants: threatened status for Southern Distinct population segment of North American green sturgeon. Fed Regist 71(67):17757–17766
Welsh AB, Blumberg M, May B (2003) Identification of microsatellite loci in lake sturgeon, Acipenser fulvescens, and their variability in green sturgeon, A. medirostris. Mol Ecol Notes 3:47–55
Wirgin A, Stabile JE, Waldman JR (1997) Molecular analysis in the conservation of sturgeons and paddlefish. Environ Biol Fishes 48:385–398
Acknowledgements
We thank the Kootenai Tribe of Idaho (Award #01633; subcontract from a grant to them from the Bonneville Power Authority) and CALFED (Award # ERP-02D-P57) for funding this work.
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Börk, K., Drauch, A., Israel, J.A. et al. Development of new microsatellite primers for green and white sturgeon. Conserv Genet 9, 973–979 (2008). https://doi.org/10.1007/s10592-007-9417-9
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DOI: https://doi.org/10.1007/s10592-007-9417-9