Skip to main content
SpringerLink
Log in

Nine microsatellite DNA primers for Hippophae rhamnoides ssp. sinensis (Elaeagnaceae)

  • Technical Note
  • Published:
Conservation Genetics Aims and scope Submit manuscript

Abstract

Hippophae rhamnoides ssp. sinensis occurs mainly in the arid regions of northwest China. The wood stands of this subspecies play an important role in maintaining the local ecosystems in these regions. In addition, the genetic characteristics are essential to understand the historical range changes of this subspecies and its morphological differentiation with other subspecies. In this study, we developed nine microsatellite loci for this subspecies for the first time. We used the combining biotin capture method to enrich AG/CT/AC/GT/CG/GTG/CCA microsatellites. Twenty-six microsatellites were isolated from the enriching library and nine of them were found to be polymorphic through screening 12 distantly distributed individuals. The number of alleles per locus ranged from three to twelve and expected heterozygosity from 0.2659 to 0.4767, respectively. We further performed cross-priming tests in another subspecies and two congeneric species. These firstly isolated loci will provide a useful tool to investigate the genetic structure of this subspecies and its morphological differentiation from the other subspecies.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Bartish IV, Jeppsson N, Nybom H, Swenson U (2002) Phylogeny of Hippophae (Elaeagnaceae) inferred from parsimony analysis of chloroplast DNA and morphology. Syst Bot 27:41–54

    Google Scholar 

  • Clarke KR, Gorley RN (2001) PRIMER v5: User Manual/Tutorial. PRIMER-E Ltd., Plymouth, pp 91

    Google Scholar 

  • Lian YS, Chen XL, Lian H (1998) Systematic classification of the genus Hippophae L. Seabuckthorn Res 1:13–23

    Google Scholar 

  • Raymond M, Rousset F (1995) GENEPOP (version 1.2): Population genetics software for exact tests and ecumenicism. J Hered 86:248–249

    Google Scholar 

  • Tang LY, Shen CM (1996) Late Cenonoic vegetation history and climatic characteristics of Qinghai-Xizang Plateau. Acta Micropalaeont Sin 13:321–337

    Google Scholar 

  • Tian CJ, Nan P, Shi SH, Chen JK, Zhong Y (2004) Molecular genetic variation in Chinese populations of three subspecies of Hippophae rhamnoides. Biochem Genet 42:259–266

    Article  PubMed  CAS  Google Scholar 

  • Zhang DY, Chen N, Yang YZ, Zhang Q, Liu JQ (2007) Development of 10 microsatellite loci for Rheum tanguticum (Polygonaceae). Conserv Genet 8 (online)

Download references

Acknowledgements

Support for this research was provided by the National Natural Science Foundation of China (30430560).

Author information

Authors and Affiliations

  1. Key Laboratory of Arid and Grassland Ecology, Lanzhou University, Lanzhou, 730000, China

    Ailan Wang, Qian Zhang, Dongshi Wan,  YanzhuoYang & Jianquan Liu

  2. Key Laboratory of Qinghai-Tibetan Plateau Biological Evolution and Adaptation, Northwest Plateau Institute of Biology, Chinese Academy of Sciences, Xining, Qinghai, China

    Jianquan Liu

Authors
  1. Ailan Wang
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Qian Zhang
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Dongshi Wan
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. YanzhuoYang
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Jianquan Liu
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jianquan Liu.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Wang, A., Zhang, Q., Wan, D. et al. Nine microsatellite DNA primers for Hippophae rhamnoides ssp. sinensis (Elaeagnaceae). Conserv Genet 9, 969–971 (2008). https://doi.org/10.1007/s10592-007-9416-x

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10592-007-9416-x

Keywords

Search

Navigation