Isolation and characterization of eight microsatellite loci for the vulnerable Hainan Eld’s deer (Cervus eldi hainanus) in China
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We report on the isolation and characterization of eight microsatellite markers in the Hainan Eld’s deer (Cervus eldi hainanus) from genomic DNA-enriched libraries. Thirty-three microsatellites were screened from the libraries, and 8 of the screened microsatellites were polymorphic. The number of observed alleles for each locus in 47 individuals ranged from 2 to 9, and the expected and observed heterozygosity was 0.141–0.792 and 0.128–0.957, respectively. Three loci (CEH-2, CEH-6 and CEH-8) of eight deviated from Hardy-Weinberg expectation and no significant linkage association was found among all these loci. These microsatellite markers provide useful tool for population genetic studies of the Eld’s deer.
KeywordsCervus eldi hainanus Cervidae Population bottleneck Microsatellite DNA Polymorphic Hainan Eld’s deer
We gratefully thank Datian National Nature Reserve, Bangxi Nature Reserve, Ganshiling Nature Reserve, Fengmu Reserve, Jinniuling Reserve, and Wenchang Reserve for providing the Hainan Eld’s deer samples. Thanks are given to Y.-L. Hao and Z.-J. Liu for their valuable technical advice and laboratory assistance. This work was financial supported by projects of the national natural science foundation of China (No. 30670241, 30470260).
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