Twenty four new microsatellite markers in two invasive pavement ants, Tetramorium sp.E and T. tsushimae (Hymenoptera: Formicidae)


Invasive species trigger biodiversity losses and alter ecosystem functioning, with life history shaping invasiveness (Sakai et al., Annu Rev Ecol Syst 32:305–332, 2001). However, pinpointing the relation of a specific life history to invasion success is difficult. One approach may be comparing congeners. The two Palearctic pavement ants, Tetramorium sp.E (widely known as T. caespitum, Schlick-Steiner et al., Mol Phylogenet Evol 40:259–273, 2006) and T. tsushimae have invaded North America (Steiner et al., Biol Invasions 8:117–123, 2006). Their life histories differ in that T. sp.E has separate single-queened colonies but T. tsushimae multi-queened colonies scattered over large areas (Sanada-Morimura et al., Insect Soc 53:141–148, 2006; Schlick-Steiner et al., Mol Phylogenet Evol 40:259–273, 2006; Steiner et al., Biol Invasions 8:117–123, 2006). Comparison of the genetic diversity in the entire native and non-native ranges will elucidate the invasion histories. Here, we present 13 and 11 microsatellites, developed for T. sp.E and T. tsushimae, respectively, and characterize all for both species.

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  1. Raymond M, Rousset F (1995) genepop Version 1.2: population genetics software for exact tests and ecumenism. J Heredity 86:248–249

    Google Scholar 

  2. Sakai AK, Allendorf FW, Holt JS et al (2001) The population biology of invasive species. Annu Rev Ecol Syst 32:305–332

    Article  Google Scholar 

  3. Sanada-Morimura S, Satoh T, Obara Y (2006) Territorial behavior and temperature preference for nesting sites in a pavement ant Tetramorium tsushimae. Insect Soc 53:141–148

    Article  Google Scholar 

  4. Schlick-Steiner BC, Steiner FM, Moder K et al (2006) A multidisciplinary approach reveals cryptic diversity in western Palaearctic Tetramorium ants (Hymenoptera: Formicidae). Mol Phylogenet Evol 40:259–273

    PubMed  Article  CAS  Google Scholar 

  5. Steiner FM, Schlick-Steiner BC, Trager JC et al (2006) Tetramorium tsushimae, a new invasive ant in North America. Biol Invasions 8:117–123

    Article  Google Scholar 

  6. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16

    PubMed  Article  CAS  Google Scholar 

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We thank N. Aktaç, M. Balint, D. Fontaneto, C. Guofa, M. Hayashi, J. Heinze, V. Kochnev, Y. Kochnev, T. Ljubomirov, D.-P. Lyu, M. Maruyama, H. Mori, J. Pontin, E. Provost, L. Qiang, H. Sakai, M. Sanetra, E. Tikhonov, M. Würmli for sample donations; S. Krumböck and A. Stradner, for technical support. FMS was supported by the Austrian Science Fund, J2642-B17.

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Correspondence to Florian M. Steiner.

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Florian M. Steiner, Wolfgang Arthofer and Birgit C. Schlick-Steiner contributed equally to this work.

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Steiner, F.M., Arthofer, W., Schlick-Steiner, B.C. et al. Twenty four new microsatellite markers in two invasive pavement ants, Tetramorium sp.E and T. tsushimae (Hymenoptera: Formicidae). Conserv Genet 9, 757–759 (2008).

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  • Invasion history
  • Invasive ants
  • Life history
  • Microsatellites
  • Tetramorium