Conservation Genetics

, Volume 9, Issue 2, pp 461–463 | Cite as

Isolation of microsatellite loci from the endemic and endangered Adriatic sturgeon (Acipenser naccarii)

  • A. Forlani
  • F. Fontana
  • L. CongiuEmail author
Technical Note


With the aim of elaborating a breeding plan on a captive stock of the highly endangered Adriatic sturgeon (Acipenser naccarii), a total of 10 polymorphic microsatellite loci were isolated from an enriched library. The results of cross amplification of additional 8 loci previously isolated from Aoxyrinchus, Afulvescens and Scaphyrinchus platorhynchus are also reported. Given the tetraploid condition of the species the genetic variability was estimated basing on the number of alleles per individuals and the average band sharing.


Acipenser naccarii Adriatic sturgeon Tetraploid FIASCO 


  1. Fontana F, Tagliavini J, Congiu L (2001) Sturgeon genetics and cytogenetics: recent advancements and perspectives. Genetica 111:359–373PubMedCrossRefGoogle Scholar
  2. Forlani A, Crestanello B, Mantovani S, Livoreil B, Zane L, Bertorelle G, Congiu L (2005) Identification and characterization of microsatellite markers in Hermann’s tortoise (Testudo hermanni, Testudinidae). Mol Ecol Notes 5:228–230CrossRefGoogle Scholar
  3. Henderson-Arzapalo A, King TL (2002) Novel microsatellite markers for Atlantic sturgeon (Acipenser oxyrinchus) population delineation and broodstock management. Mol Ecol Notes 2:437–439CrossRefGoogle Scholar
  4. McQuown EC, Sloss BL, Sheehan RJ, Rodzen J, Tranah G, May B (2000) Microsatellite analysis of genetic variation in sturgeon: new primer sequences for Scaphirhynchus and Acipenser. Trans Am Fish Soc 129:1380–1388CrossRefGoogle Scholar
  5. Vos P, Hogers R, Bleeker M, Reijans M, van de Lee T, Hornes M (1995) AFLP: a new technique for DNA fingerprinting. Nucleic Acids Res 23:4407–4414PubMedCrossRefGoogle Scholar
  6. Welsh AB, Blumberg M, May B (2003) Identification of microsatellite loci in lake sturgeon, Acipenser fulvescens, and their variability in green sturgeon, A. medirostris. Mol Ecol Notes 3:47–55CrossRefGoogle Scholar
  7. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol Ecol 11:1–16PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  1. 1.Dipartimento di BiologiaUniversità di FerraraFerraraItaly
  2. 2.Dipartimento di BiologiaUniversità di PadovaPadovaItaly

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