Abstract
Ring-tailed lemur (Lemur catta) is the only species in the Genus Lemur, distributed in the deciduous and spiny forests of southwestern Madagascar. This species is listed as endangered due to the loss and fragmentation of its natural habitat, a consequence of deforestation. Twenty-one nuclear microsatellite loci were isolated from a genomic DNA derived from a free-ranging ring-tailed lemur population from the Tsimanampetsotsa National Park, Madagascar. We report various parameter estimates and measures to establish the utility of this marker suite as screened among individuals this single forest fragment.
This is a preview of subscription content, log in via an institution to check access.
References
Dieringer D, Schlötterer C (2003) Microsatellite analyzer (MSA): a platform independent analysis tool for large microsatellite data sets. Available: http://www.i122server.vu-wien.ac.at/MSA/info.html/MSA_info.html. Mol Ecol Notes 3:167–169
Garbutt N (1999) Mammals of Madagascar. Yale University Press, New Haven and London
Goudet J (1995) FSTAT, a computer program to test F-statistics. J Hered 86:485–486
Goudet J (2001) FSTAT, a program to estimate and test gene diversities and fixation indices (version 2.9.3). Available: http://www.unil.ch/izea/softwares/fstat.html. Updated from Goudet (1995)
Groves CP (2001) Primate Taxonomy. Smithsonian Institution Press, Washington, DC, USA
Hillis DM, Mable BK, Larson A, Davis SK, Zimmer EA (1996) Nucleic acids IV: sequencing and cloning. In: Hillis DM, Moritz C, Zimmer EA (eds), Molecular Systematics, 2nd edn. Sinauer Associates Inc, Sunderland MA, pp 321–381
Jenkins PD (1987) Catalogue of Primates in the British Museum (Natural History) and elsewhere in the British Isles Part IV: Suborder Strepsirrhini, including the subfossil Madagascan lemurs and Family Tarsiidae. British Museum (Natural History), London
Kandpal RP, Kandpal G, Weissman SM (1994) Construction of libraries enriched for sequence repeats and jumping clones, and hybridization selection for region-species markers. Proc Natl Acad Sci USA 91:88–92
Longmire JL, Gee GF, Hardekoff CL, Mark GA (1982) Establishing paternity in whooping cranes (Grus americana) by DNA analysis. Auk 109:522–529
Marshall TC, Slate J, Kruuk LEB, Pemberton JM (1998) Statistical confidence for likelihood-based paternity inference in natural populations. Mol Ecol 7(5):639–655
Mittermeier RA, Tattersall I, Konstant WR, Meyers D, Mast R (1994) Lemurs of Madagascar. Conservation International, Washington DC, pp 160–166
Mittermeier RA, Konstant WR, Hawkins F, Louis EE, Langrand O, Ratsimbazafy J, Rasoloarison R, Ganzhorn JU, Rajaobelina S, Tattersall I, Meyers DM (2006) Lemurs of Madagascar 2nd Edition. Conservation International Tropical Field Guide Series, Washington DC, pp 391–403
Moraga-Amador D, Farmerie BA, Brazeau D, Clark G (2001) “Tools for Developing Molecular Markers.” Interdisciplinary Center for Biotechnology Research Laboratory Manual. The University of Florida Gainesville, Florida, pp 11–65. Available: http://www.biotech.ufl.edu/WorkshopsCourses/Word_files/Molecular%20Markers%20Manual%202001.doc
Prichard JK, Stephens M, Donnelly P (2000) Inference of population structure using multilocus genotype data. Genetics 155:945–959
Raymond M, Rousset F (1995) GENEPOP (version 1.2): population genetics software for exact tests and ecuminicisms. Available: http://www.wbiomed.curtin.edu.au/genepop/ J Hered 86:248–249
Sambrook J, Fritch EF, Maniatus T (1989) Molecular cloning: a laboratory manual 2nd edn. Cold Spring Harbor Press, New York
Slate J, Marshall TC, Pemberton JM (2000) A retrospective assessment of the accuracy of the paternity inference program CERVUS. Available: http://www.helios.bto.ed.ac.uk/evolgen/cervus/cervus.html. Mol Ecol 9(6):801–808
Van Oosterhaut C, Hutchinson WF, Wills DPM, Shipley P (2004) MICRO-CHECKER: software for identifying and correcting genotyping errors in microsatellite data. Available: http://www.microchecker.hull.ac.uk/. Mol Ecol Notes 4:535–538
Yoder AD, Irwin JA, Goodman SM, Rakotoarisoa SV (2000) Genetic tests of the taxonomic status of the ring-tailed lemur (Lemur catta) from the high mountain zone of the Andringitra Massif, Madagascar. J Zool Lond 252:1–9
Acknowledgements
The authors wish to acknowledge the generosity of Bill and Berniece Grewcock for their support of student interns. This research was also supported by grants from the Ahmanson Foundation, which have provided the laboratory with three ABI automated DNA sequencers. We graciously thank the Theodore F. and Claire M. Hubbard Family Foundation for their support of the Henry Doorly Zoo/Madagascar Biodiversity and Biogeography Project. This project would not have been possible without the support of the staff, guides, drivers, and porters of the Institute for Conservation of Tropical Environments, Madagascar (ICTE-MICET), the Association Nationale pour la Gestion des Aires Protégées (ANGAP) and the Ministère des Eaux et Foret, Madagascar.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Zaonarivelo, J.R., Andriantompohavana, R., Shore, G.E. et al. Characterization of 21 microsatellite marker loci in the ring-tailed lemur (Lemur catta). Conserv Genet 8, 1209–1212 (2007). https://doi.org/10.1007/s10592-006-9259-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s10592-006-9259-x