Abstract
The hexaploid herbaceous species Primula glaucescens Moretti and Primula spectabilis Tratt. (2n = 6x = 66) are two endemics of the Italian Pre-Alps protected by national and international laws. In order to plan conservation strategies for natural populations and to study the influence of the latest glaciation on them we developed a set of microsatellites markers for the endangered Primula species: ten primer pairs allowed analysis of polymorphic disomic loci in P. glaucescens samples and seven of them also amplified polymorphic disomic markers in P. spectabilis.
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Acknowledgements
The research formed a part of the Life Nature 2000 Project “Integrated management of Insubric Prealpine habitats” (LIFE00NAT/IT/7258). The research activities and the fellowship of Davide Perini was supported: (i) by Centro Flora Autoctona della Regione Lombardia, Consorzio Parco Monte Barro (Lecco-Italy) at the Università degli Studi dell’ Insubria, (ii) by a Marie Curie Fellowship of the European Community programme Human Potential under contract number HPMT-CT-2001-00272 at the University of Newcastle upon Tyne (UK).
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Kirsten Wolff and Giorgio Binelli contributed equally to the paper
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Perini, D., Meloni, M., Wolff, K. et al. Isolation and characterization of microsatellites in Primula glaucescens Moretti and their cross-species amplification in P. spectabilis Tratt.. Conserv Genet 8, 1263–1266 (2007). https://doi.org/10.1007/s10592-006-9258-y
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DOI: https://doi.org/10.1007/s10592-006-9258-y