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Isolation and characterization of microsatellite loci in Cryptocarya chinensis in lower subtropical China

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Abstract

We report on the isolation and characterization of eight microsatellite markers from repetitive DNA enriched libraries for Cryptocarya chinensis from lower subtropical China. These are the first microsatellite loci reported for Cryptocarya species. The number of alleles ranged from three to nine. Observed and expected heterozygosities ranged from 0.0518 to 0.9910 and 0.5241 to 0.7935 for polymorphic loci, respectively. These markers will allow analyses of the baseline genetic variability and population structure of C. chinensis to enrich our scientific understanding of forest fragmentation on genetic health of this species and provide strategies for effective conservation and management in this area.

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Acknowledgements

We thank San-Hong Gao, Sheng-Ni Tian, Ci-You Zhu, Tian-Min Wu and Wei-Nan Ye for helping collect sampling and/or assistance in the laboratory. Thank Dr Carolyne Bardeleben for reviewing and helping improving English writing. This work was supported by Natural Science Foundation of China (No. 30300055), Natural Science Foundation of Guangdong Province (No. 031264) and Science Utility Project of Guangdong Province (No. 2005B60301001).

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Authors and Affiliations

  1. Guangdong Key Laboratory of Digital Botanical Garden, South China Botanical Garden, Chinese Academy of Sciences, Guangzhou, 510650, P.R. China

    Zheng-Feng Wang, Sheng-Lei Fu & Hai Ren

  2. The Key Laboratory of Gene Engineering of Ministry of Education, Sun Yat-Sen University, Guangzhou, 510275, P.R. China

    Gang Li

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  1. Zheng-Feng Wang
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  2. Gang Li
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  3. Sheng-Lei Fu
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  4. Hai Ren
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Correspondence to Gang Li.

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Wang, ZF., Li, G., Fu, SL. et al. Isolation and characterization of microsatellite loci in Cryptocarya chinensis in lower subtropical China. Conserv Genet 8, 1235–1237 (2007). https://doi.org/10.1007/s10592-006-9236-4

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  • DOI: https://doi.org/10.1007/s10592-006-9236-4

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