Abstract
Eleven novel polymorphic microsatellite loci developed from a microsatellite enriched genomic library, are presented for the Australian tree frog Litoria peronii. We screened 29 individuals from a single population and detected high levels of polymorphism for all 11 loci with the number of alleles/locus ranging from 9 to 24. Values of expected and observed heterozygosities ranged from 0.789–0.955 and 0.207–1.00, respectively. These microsatellite markers should prove useful in determining levels of genetic diversity, measuring gene flow and migration, assigning individuals to their most likely population of origin, and in the assignment of paternity.
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Alford RA, Richards SJ (1999) Global amphibian declines: a problem in applied ecology. Annu Rev Ecol Syst 30: 133–165
Alford RA, Dixon PM, Pechmann JHK (2001) Ecology—global amphibian population declines. Nature 412: 499–500
Armour JA, Neumann R, Gobert S, Jeffreys AJ (1994) Isolation of human simple repeat loci by hybridization selection. Hum Mol Genet 3: 599–565
Cogger H (2000) Reptiles and amphibians of Australia, 6th edn. Reed New Holland Publishers, Sydney
Cushman SA (2006) Effects of habitat loss and fragmentation on amphibians: a review and prospectus. Biol Conserv 128: 231–240
Excoffier L, Laval G, Schneider S (2005) Arlequin ver. 3.0: an integrated software package for population genetics data analysis. Evol Bioinformat Online 1: 47–50
Gautschi B, Tenzer I, Müller JP, Schmid B (2000a). Isolation and characterization of microsatellite loci in the bearded vulture (Gypaetus barbatus) and crossamplification in three Old World vulture species. Mol Ecol 9: 2193–2195
Gautschi B, Widmer A, Koella J (2000b). Isolation and characterization of microsatellite loci in the dice snake (Natrix tessellata). Mol Ecol 9: 2191–2193
Houlahan JE, Findlay CS, Schmidt BR, Meyer AH, Kuzmin SL (2000) Quantitative evidence for global amphibian population declines. Nature 404: 752–755
Jehle R, Arntzen JW (2002) Microsatellite markers in amphibian conservation genetics. Herpetol J 12: 1–9
Raymond M, Rousset F (1995) Genepop (Version 1.2): Population genetics software for exact tests and ecumenicism. J Hered 86: 248–249
Rice WR (1989) Analyzing tables of statistical tests. Evolution 43:223–225
Sambrook J, Fritsch EF, Maniatas T (1989) Molecular cloning: a laboratory manual, 2nd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, New York
Stuart SN, Chanson JS, Cox NA, Young BE, Rodrigues ASL, Fischman DL, Waller RW (2004) Status and trends of amphibian declines and extinctions worldwide. Science 306: 1783–1786
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This work was supported by an Australian Research Council Discovery Grant to Mats Olsson (Grant # DP0559867).
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Sherman, C.D.H., Olsson, M. Polymorphic microsatellite loci in the Australian tree frog, Litoria peronii . Conserv Genet 8, 999–1001 (2007). https://doi.org/10.1007/s10592-006-9218-6
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DOI: https://doi.org/10.1007/s10592-006-9218-6