Genetic isolation and evolutionary history of oases populations of the Baja California killifish, Fundulus lima
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The Baja California killifish, Fundulus lima, is found in six desert oases of the southern Baja California Peninsula, Mexico. The recent introduction of exotic fishes, particularly redbelly tilapia, have impacted the ecology of Fundulus lima such that it is now endangered. Plans of relocating F. lima to bodies of freshwater that are free of exotics have been proposed, however little is know about the genetic identity of the current populations. In this study, we examined the mitochondrial control region of F. lima samples from 4 oases, and in addition, compared these samples to their sister species, the California killifish F.␣parvipinnis. Using a combination of phylogenetic and coalescent approaches, we were able to determine that the two subspecies of the California killifish, F. p.␣brevis, and F. p. parvipinnis, and F. lima form an unresolved trichotomy that diverged between 200,000 years and 400,000 years ago. The one F. lima individual that we were able to collect in the southernmost oasis grouped with the southern subspecies of the California killifish, F. parvipinnis brevis. In contrast, we found that the 3 northern oases grouped together in a “Fundulus lima” clade. Each oasis is genetically distinct, yet there is no evidence of a␣marked genetic bottleneck in any populations (Haplotype diversity between 0.5 and 0.8). Future relocation plans will therefore need to be done cautiously to preserve the genetic identity of the original populations.
KeywordsFundulus lima Fundulus parvipinnis Baja California Desert fishes Control region
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We would like to thank A. Antuna and Q.␣Talamantes for their help in the fish sampling, Vera Domingues and Drew Talley for discussion This research was supported by UC MEXUS.
- Avise JC (1994) Molecular markers, natural history and evolution. Chapman and Hall, New YorkGoogle Scholar
- Avise JC (2000) Phylogeography: the history and formation of species. Harvard University Press, Cambridge, MassGoogle Scholar
- Bernardi G (1997) Molecular phylogeny of the Fundulidae (Teleostei, Cyprinodontiformes) based on the cytochrome b gene. In: Kocher T, Stepien C (eds) Molecular Systematics of Fishes. Academic Press, pp 185–194Google Scholar
- Dumke RH (1976) Geographic variation in the California killifish, Fundulus parvipinnis Girard (Family Cyprinodontidae). M.Sc. Thesis. CSU FullertonGoogle Scholar
- Fagan WF, Aumann C, Kennedy CM, Unmack PJ (2005) Rarity, fragmentation, and the scale of dependence of extinction risk in desert fishes. Ecology 86:34–41Google Scholar
- Farris JS (1968) The evolutionary relationships between the species of killifish genera Fundulus and Profundulus (Teleostei: Cyprinodontidae). Unpublished Ph.D. dissertation, University of Michigan, Ann Arbor, MIGoogle Scholar
- McCune AR, Lovejoy NR (1998) The relative rate of sympatric and allopatric speciation in fishes: tests using DNA sequence divergences between sister species and among clades. In: Howard DJ, Berlocher SH (eds) Endless forms: species and speciation. Oxford University Press, New YorkGoogle Scholar
- Miller DJ, Lea RN (1972) Guide to coastal marine fishes of California. Fish Bulletin #157. Department of Fish and Game. Sacramento, CaliforniaGoogle Scholar
- Sambrook J, Fritsch EF, Maniatis T (1989) Molecular cloning: a laboratory manual, 2nd ed. Cold Spring Harbor Laboratory, Cold Spring Harbor, NYGoogle Scholar
- Shimodaira H, Hasegawa M (1999) Multiple comparisons of log-likelihoods with applications to phylogenetic inference. Mol Biol Evol 16:1114–1116Google Scholar
- Swofford DL (2003) PAUP*. Phylogenetic Analysis Using Parsimony (*and Other Methods). Version 4.0b10. Sinauer Associates, Sunderland, MassachusettsGoogle Scholar
- Wiley EO (1986) A study of evolutionary relationships of Fundulus topminnows (Teleostei: Fundulidae). Am Zool 26:121–130Google Scholar