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Genetic variation at MHC, mitochondrial and microsatellite loci in isolated populations of Brown trout (Salmo  trutta)

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Abstract

We have studied levels and distribution of genetic variation in nine isolated populations of Brown trout in NW Spain. In the present study, we have tried to test the importance of preservation of genetic variability for the survival of a set of isolated Brown trout (Salmo trutta) populations from the same river drainage. We screened genetic variation in three different markers, mitochondrial, microsatellites and Major Histocompatibility Complex (MHC), presumed to be under different selective pressures. Overall, genetic diversity varied considerably across populations and the distribution of genetic variation was similar at MHC and microsatellites; highly polymorphic populations at the microsatellite loci were also highly polymorphic at the MHC. We also observed high levels of differentiation among populations. Although we found evidence suggesting that balancing selection has influenced the long term evolution of the MHC, genetic drift seems to have eroded the effect of selection, becoming the predominant evolutionary force shaping genetic variation in some of the smaller populations. Despite current lack of variation at the MHC, these small populations seem to have remained viable for a long time.

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Acknowledgements

We are very grateful to José Manuel Cano, David Alvarez and Alfredo González Nicieza for generously providing the samples used here, and for supplying concrete details about the rivers studied. We appreciate comments on the manuscript from Armando Caballero, Antonio Carvajal-Rodríguez, Humberto Quesada and Emilio Rolán. Pilar Alvariño and Nieves Santamaria assisted with technical support. We are thankful for the help provided by José Luis Martínez and Eva García-Vázquez’s lab. Jose L Campos is supported by a grant from Xunta de Galicia.

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  1. Departamento de Bioquímica, Genética e Inmunología, Facultad de Biología, Universidad de Vigo, 36310, Vigo, Spain

    José L. Campos, David Posada & Paloma Morán

  2. Area de Genética, Edificio de Ciencias Experimentales, Campus Universitario, 36310, Vigo, Spain

    José L. Campos

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  1. José L. Campos
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Correspondence to José L. Campos.

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Campos, J., Posada, D. & Morán, P. Genetic variation at MHC, mitochondrial and microsatellite loci in isolated populations of Brown trout (Salmo  trutta). Conserv Genet 7, 515–530 (2006). https://doi.org/10.1007/s10592-005-9063-z

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