Isolation of polymorphic microsatellite loci for the study of habitat fragmentation in the harvester ant Messor structor


Microsatellite sequences of the harvester ant Messor structor were identified by use of an enriched genomic library. Populations from Austria, Germany and Croatia were screened and 4–21 alleles were identified within seven microsatellite loci. Cross species amplification was evaluated for three other Messor species.

This is a preview of subscription content, log in to check access.


  1. Chapman R, Bourke AFG (2001) The influence of sociality on the conservation biology of social insects. Ecol. Letters 4: 650–662

    Article  Google Scholar 

  2. Crozier RH, Oldroyd BP, Tay WT, Kaufmann BE, Johnson RN, Carew ME, Jennings KM (1997) Molecular advances in understanding social insect population structure. Electrophoresis 18: 1672–1675

    Article  PubMed  CAS  Google Scholar 

  3. Gyllenstrand N, Seppä P (2003) Conservation genetics of the wood ant, Formica lugubris, in a fragmented landscape. Mol. Ecol. 12: 2931–2940

    Article  PubMed  CAS  Google Scholar 

  4. Raymond M, Rousset F (1995) GENEPOP Version 1.2: Population genetics software for exact tests and ecumenism. J. Heredity 86: 248–249

    Google Scholar 

  5. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. Cold Spring Harbor Laboratory Press, New York

    Google Scholar 

  6. Schlick-Steiner BC, Steiner FM, Schödl S (2003) Rote Listen ausgewählter Tiergruppen Niederösterreichs – Ameisen (Hymenoptera: Formicidae), 1. Fassung 2002. Amt der NÖ Landesregierung, Abteilung Naturschutz, St. Pölten

    Google Scholar 

  7. Steiner FM, Schlick-Steiner BC (2002) Einsatz von Ameisen in der naturschutzfachlichen Praxis. Begründungen ihrer vielfältigen Eignung im Vergleich zu anderen Tiergruppen. Naturschutz und Landschaftsplanung 34: 5–13

    Google Scholar 

  8. Zane L, Bargelloni L, Patarnello T (2002) Strategies for microsatellite isolation: a review. Mol. Ecol. 11: 1–16

    Article  PubMed  CAS  Google Scholar 

Download references


To B. Becker, A. Buschinger, E. Christian, R.␣Crozier, K. Gómez, G. Heller, R. Huertas, K.␣Moder, K. Sefc, B. Seifert, M. Stachowitsch. This work was funded by the Austrian Science Foundation P16794-B06.

Author information



Corresponding author

Correspondence to W. Arthofer.

Additional information

W. Arthofer, B. C. Schlick-Steiner and F. M. Steiner contributed equally to this work.

Rights and permissions

Reprints and Permissions

About this article

Cite this article

Arthofer, W., Schlick-Steiner, B.C., Steiner, F.M. et al. Isolation of polymorphic microsatellite loci for the study of habitat fragmentation in the harvester ant Messor structor . Conserv Genet 6, 859–861 (2005).

Download citation

Key words

  • conservation
  • habitat fragmentation
  • Messor structor
  • microsatellites
  • social insects