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Mapping protein signal pathway interaction in sarcoma bone metastasis: linkage between rank, metalloproteinases turnover and growth factor signaling pathways

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Abstract

We applied reverse phase protein microarrays technology to map signal pathway interactions in a discovery set of 34 soft tissue sarcoma (STS) bone metastases compared to healthy bone. Proteins associated with matrix remodeling (MMP), adhesion (FAK Y576/577, Syndecan-1), and growth/survival (IGF1R Y1135/1136, PI3K, EGFR) were elevated in metastasis compared to normal bone. Linkage between Syndecan-1, FAK Y576/577, Shc Y317, and EGFR, IGF Y1135/1136, PI3K/AKT was a prominent feature of STS bone metastasis. Elevated linkage between RANKL and 4EBP1 T37/46, EGFR, IGF1R Y1135/1136, Src Y41, Shc Y317, PI3Kp110γ was associated with short survival. Finally, we tested the hypothesis that signal pathway proteins augmented in the STS bone metastasis may provide clues to understand the subset of primary STS that metastasize. The most representative molecules identified in the discovery set were validated on an independent series of 82 primary STS by immunohistochemistry applied to a tissue microarray. The goal was to correlate the molecular profile in the primary tumors with a higher likelihood of metastasis. Elevation of activated kinase substrate endpoints IRS1 S612, 4EBP1 T37/46, FAK Y576/577 and loss of Fibronectin, were associated with a higher likelihood of metastases. These data indicate that the linkage between matrix remodeling, adhesion, and growth signaling may drive STS metastasis and can be the basis for prognostic and therapeutic strategies.

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Acknowledgments

We are grateful to Ms. Cristina Ghinelli for the graphics, Dr. Elettra Pignotti for statistical analysis, Dr. Alba Balladelli for editing and the Rizzoli Institute surgical pathology technicians. This work was supported by: George Mason University and the Italian Istituto Superiore di Sanità in the framework of the Italy/USA cooperation agreement between U.S department of Health and Human Service, George Mason University and the Italian Ministry of Public Health; Chiara Novello was supported by Grant 13748 from Fondazione Italiana per la Ricerca sul Cancro (FIRC).

Conflict of interest

The authors declare that they have no conflict of interest.

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Authors and Affiliations

  1. Laboratory of Experimental Oncology, Rizzoli Orthopedic Institute, Via di Barbiano 1/10, 40136, Bologna, Italy

    Amalia Conti, Antonella Chiechi, Giovanna Magagnoli, Chiara Novello, Laura Pazzaglia, Irene Quattrini, Piero Picci & Maria Serena Benassi

  2. Center for Applied Proteomics and Molecular Medicine, George Mason University, Manassas, VA, USA

    Amalia Conti, Virginia Espina, Antonella Chiechi & Lance A. Liotta

Authors
  1. Amalia Conti
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  2. Virginia Espina
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  3. Antonella Chiechi
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  4. Giovanna Magagnoli
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  5. Chiara Novello
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  6. Laura Pazzaglia
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  7. Irene Quattrini
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  8. Piero Picci
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  9. Lance A. Liotta
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  10. Maria Serena Benassi
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Correspondence to Maria Serena Benassi.

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Conti, A., Espina, V., Chiechi, A. et al. Mapping protein signal pathway interaction in sarcoma bone metastasis: linkage between rank, metalloproteinases turnover and growth factor signaling pathways. Clin Exp Metastasis 31, 15–24 (2014). https://doi.org/10.1007/s10585-013-9605-6

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  • DOI: https://doi.org/10.1007/s10585-013-9605-6

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