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Major but not minor hepatectomy accelerates engraftment of extrahepatic tumor cells

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Abstract

Background

The effect of hepatectomy and hepatic regeneration on intra- and extrahepatic tumor growth is still controversially discussed. Herein we studied the effect of minor (30%) or major (70%) hepatectomy on engraftment of extrahepatic tumor cells, and the role of tumor neovascularization and tumor cell migration.

Methods

Green fluorescent protein (GFP)-transfected CT26.WT colorectal cancer cells were implanted in dorsal skinfold chambers of syngeneic BALB/c mice. Animals underwent 30% (30%Phx, n = 8) or 70% hepatectomy (70%Phx, n = 8). Sham-operated animals served as controls (n = 8). Angiogenesis and neovascularization as well as tumor cell migration, proliferation and growth were studied over 14 days using intravital fluorescence microscopy, histology and immunohistochemistry.

Results

After both minor and major hepatectomy tumor proliferating cell nuclear antigen (PCNA) expression increased significantly (P < 0.05) when compared with nonhepatectomized controls. However, only major but not minor hepatectomy accelerated neovascularization (P < 0.05) and tumor cell migration (P < 0.05). This was associated with a significantly (P < 0.05) enhanced tumor growth after 70%Phx when compared with 30%Phx and controls. The rate of apoptotic cell death was not affected by major or minor hepatectomy.

Conclusion

Regeneration after major hepatectomy accelerates extrahepatic tumor cell engraftment, most probably by acceleration of neovascularization and induction of tumor cell migration.

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Acknowledgments

We appreciate the excellent technical assistance of Christina Marx and Janine Becker. This study was supported by grants of the Research Committee and the Medical Faculty of the University of Saarland (HOMFOR-A/2003/1).

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Authors and Affiliations

  1. Department of General, Visceral, Vascular and Pediatric Surgery, University of Saarland, 66421, Homburg/Saar, Germany

    Kathrin Rupertus, Otto Kollmar, Bastian Junker & Martin K. Schilling

  2. Institute for Clinical and Experimental Surgery, University of Saarland, 66421, Homburg/Saar, Germany

    Claudia Scheuer & Michael D. Menger

Authors
  1. Kathrin Rupertus
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  2. Otto Kollmar
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  3. Claudia Scheuer
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  4. Bastian Junker
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  5. Michael D. Menger
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  6. Martin K. Schilling
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Correspondence to Otto Kollmar.

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Rupertus, K., Kollmar, O., Scheuer, C. et al. Major but not minor hepatectomy accelerates engraftment of extrahepatic tumor cells. Clin Exp Metastasis 24, 39–48 (2007). https://doi.org/10.1007/s10585-006-9054-6

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  • DOI: https://doi.org/10.1007/s10585-006-9054-6

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