Skip to main content
SpringerLink
Log in

Elemental composition of leukocyte subfractions

  • Session 2: Nuclear Analytical Methods
  • Published:
Czechoslovak Journal of Physics Aims and scope

Abstract

The aim of this preliminary investigation was to determine the elemental concentration of various subfractions of leukocytes in a normal subject. Little work has been published on the elemental composition of these subfractions. First, a reliable technique for separation of these subfractions had to be established so that it could be applied to the determination of elemental concentrations in leukocyte subfractions from patients undergoing heart bypass surgery. Instrumental neutron activation analysis (INAA) utilising short irradiation and counting was the technique employed. Various washing media were examined during the separation of the leukocyte subfractions, for contamination of these small samples of peripheral blood mononuclear cells (PBMC) and polymorphonuclearcytes (PMN). Early results showed Mg and Se were present in these subfractions. Possibilities for further work are also discussed.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Kruse-Jarres J. D. and Rükgauer M.: Journal of Trace Elements in Medicine and Biology 14 (2000) 21.

    Article  Google Scholar 

  2. Mielcarz G., et al.: Journal of Trace Elements in Medicine and Biology 15 (2001) 31.

    Article  Google Scholar 

  3. Ferrante, A., Thong Y. H.: Journal of Immunological Methods 36 (1980) 109.

    Article  Google Scholar 

  4. Whitehouse R. C., Prasad A. S., Rabbani P. I. and Cossack Z. T.: Clinical Chemistry 28 (1982) 475.

    Google Scholar 

  5. Bonnet, M. C., Thomas J. P., Betuel H. and Fallavier M.: Journal of Ion Beam Surface Layers Analysis 1 (1976) 785.

    Google Scholar 

  6. Spyrou N.: Journal of Radioanalytical Chemistry 61 (1981) 211.

    Article  Google Scholar 

  7. Mehta A. B. and Hoffbrand A. V.: Haematology at a Glance, Blackwells Sciences Ltd, London, 2000, 16.

    Google Scholar 

  8. Al-Bader A., et al.: Cardiovascular Surgery 6 (1998) 406.

    Article  Google Scholar 

  9. Leung, F. Y.: The Journal of Nutritional Biochemistry 9 (1998) 304.

    Article  Google Scholar 

  10. Admans L. L., Beasley D., Spyrou N. M.: University of Surrey Ion Beam Centre Eleventh Annual User Workshop, March 2002.

Download references

Author information

Authors and Affiliations

  1. Department of Physics, School of Physics and Chemistry, University of Surrey, GU2 7XH, Guildford, Surrey, UK

    L. L. Admans & N. M. Spyrou

Authors
  1. L. L. Admans
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. N. M. Spyrou
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Our thanks go to Dr. Jennifer Tooze for use of the Department of Haematology facilities at St George’s Hospital, London, UK. Dr. Waleed J. Altaf, Department of Physics, University of Umm Al-Qura, Makkah, Saudi Arabia for his assistance.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Admans, L.L., Spyrou, N.M. Elemental composition of leukocyte subfractions. Czech J Phys 53 (Suppl 1), A195–A200 (2003). https://doi.org/10.1007/s10582-003-0025-8

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10582-003-0025-8

Keywords

Search

Navigation