Global epigenetic changes of histone modification under environmental stresses in rice root
- 99 Downloads
Abiotic stresses are non-living factors with negative morphological and physiological effects on living organisms. Substantial evidence exists that gene expression changes during plant cell growth are regulated by chromatin reconfiguration and histone modification. Several types of histone modifications are dramatically transformed in stress-responsive gene regions under drought stress conditions. Environmental stresses also cause the root apical meristem (RAM) region to decelerate root growth. In this study, we investigated how quantitative changes in epigenetic markers in this region influence rice morphology and physiology. Both iron and salinity treatments changed the epigenetic landscape from euchromatic to heterochromatic according to heterochromatin (H3K9me2) and euchromatin (H3K4me) markers, especially in the proximal meristem region. Moreover, supplementation with external abscisic acid (ABA) was able to mimic the effect of environmental stresses on global epigenetic changes. In contrast, the addition of external auxin (IAA) to rice under saline conditions affected heterochromatin formation without influencing euchromatin transformation. Chromatin dynamics is therefore believed to be directly connected to plant growth regulator signaling. We discuss insights into the role of plant growth regulators: ABA and IAA, peroxide signaling, and their effects on the global epigenetic change of histone modification under abiotic stresses.
KeywordsABA IAA Root apical meristem Iron stress Salinity stress H3K9me2 H3K4me2
Root apical meristem
Histone H3 dimethylation at lysine 9
Histone H3 dimethylation at lysine 4
This work was supported by the SmartD program (IAARD-Indonesian Ministry of Agriculture) to AP, and JSPS and CAS under the Japan-Czech Research Cooperative Program to NO. We thank Edanz Group (www.edanzediting.com/ac) for editing the English text of a draft of this manuscript.
NO conceived of the study, participated in its design and coordination, and drafted the manuscript. AP carried out collected experiments, assembly of data, performed statistical analysis studies, and drafted the manuscript. WE prepared the rice materials and assisted with data interpretation. All authors read and NO approved the final manuscript.
- Cutler SR, Rodriguez PL, Finkelstein RR, Abrams SR (2010) Abscisic acid: emergence of a core signaling network. In Annual Review of Plant Biology 61:651–679Google Scholar
- Gulnaz A, Iqbal J, Azam F (1999) Seed treatment with growth regulators and crop produktivity. II. Response of critical growth stages of wheat (Triticum aestivum L.) under salinity stress. Cereal Res 27:419–426Google Scholar
- Kim J-M, Sasaki T, Ueda M, Sako K, Seki M (2015) Chromatin changes in response to drought, salinity, heat, and cold stresses in plants. Front Plant Sci 6:1–12Google Scholar
- Lee SC, Luan S (2012) ABA signal transduction at the crossroad of biotic and abiotic stress responses. Plant Cell Environ 35:53–60Google Scholar
- Liszkay A, van der Zalm E, Schopfer P (2004) Production of reactive oxygen intermediates (O2˙−, H2O2, and ˙OH) by maize roots and their role in wall loosening and elongation growth. Plant Physiol 136:3114–3123Google Scholar