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Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species

Abstract

Interstitial telomeric repeats (ITRs) were reported in a number of animal and plant species. Most ITRs are organized as short tandem arrays and are likely evolutionary relics derived from chromosomal rearrangements and DNA repairs. However, megabase-sized ITR arrays were reported in Solanum species. Here, we report a fluorescence in situ hybridization (FISH) survey of ITRs in all representative diploid Solanum species, including potato, tomato, and eggplant. FISH revealed massive amplification of ITRs in the centromeric regions of chromosomes from the Solanum species containing the B and P genomes. A significant proportion of the ITR FISH signals was mapped within the primary constrictions of the pachytene chromosomes of Solanum pinnatisectum (B genome). In addition, some ITR sites overlapped with St49, a satellite repeat enriched in centromeric DNA sequences associated with CENH3 nucleosomes, in both A and B genome Solanum species. These results show that some ITR subfamilies have been amplified and invaded in the functional centromeres of chromosomes in Solanum species.

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Abbreviations

ITRs:

Interstitial telomeric repeats

FISH:

Fluorescence in situ hybridization

BAC:

Bacterial artificial chromosome

DAPI:

4′,6-Diamidino-2-phenylindole

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Acknowledgments

We thank Dr. Christian Bachem, Wageningen University, the Netherlands, for providing the potato BAC clones. This work was supported by grant 2009CDA085 from the Natural Science Foundation of Hubei Province and grant 2006AA100107 from the National High-Tech Program of China to L.J., and by grant IOS1237969 from the National Science Foundation (USA) to J.J. G.A.T. was supported by a CAPES fellowship from the Brazilian Ministry of Education, Brazil.

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Correspondence to Conghua Xie.

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Responsible Editor: Hans de Jong.

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He, L., Liu, J., Torres, G.A. et al. Interstitial telomeric repeats are enriched in the centromeres of chromosomes in Solanum species. Chromosome Res 21, 5–13 (2013). https://doi.org/10.1007/s10577-012-9332-x

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  • DOI: https://doi.org/10.1007/s10577-012-9332-x

Keywords

  • interstitial telomeric repeats
  • centromere
  • potato
  • Solanum
  • fluorescence in situ hybridization