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Advanced comparative cytogenetic analysis of X chromosomes in river buffalo, cattle, sheep, and human

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Abstract

Based on a recently generated comprehensive gene map for Ovis aries chromosome X (OARX) with an approximately even locus distribution, we assigned selected bacterial artificial chromosome (BAC) probes corresponding to these OARX loci to Bubalus bubalis (BBU) and Bos taurus (BTA) by comparative fluorescence in-situ hybridization (FISH) to improve cytogenetically the X chromosome maps in these species. Twenty-five added loci in BBUX and BTAX, respectively, contribute to a more detailed description of the cytogenetic organization of these chromosomes. Further seven loci were identified in OARX and two DNA probes were assigned to X and Y chromosomes in river buffalo, cattle, and sheep, respectively, and thus identified loci in the pseudoautosomal region. The additional assignments double the number of cytogenetic loci in BBUX and increase their number in BTAX and OARX. The larger quantity of cytogenetic anchors allows a more precise morphological comparison of bovid X chromosomes among each other and with the Homo sapiens (HSA) X chromosome. The anchor loci confirm and refine syntenic fragments in HSAX and identify several evolutionary breakpoints between the compared chromosomes. The cytogenetic assignments in BBUX, BTAX, and OARX represent useable anchors for the ongoing genome sequence assembly in Bovidae.

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Abbreviations

BAC:

Bacterial artificial chromosome

BBU:

Bubalus bubalis

BES:

BAC end sequence

BLAST:

Basic local alignment search tool

BTA:

Bos taurus

CSKBB:

Committee for Standardized Karyotype of Bubalus bubalis

DABCO:

1,4-Diazabicyclo[2.2.2]octan

FISH:

Fluorescence in-situ hybridization

FITC:

Fluorescein isothiocyanate

HSA:

Homo sapiens

ISCNDB:

International System for Chromosome Nomenclature of Domestic Bovids

ISCN:

International System for Human Cytogenetic Nomenclature

OAR:

Ovis aries

OGA:

Ovine Genome Assembly

PAB:

Pseudoautosomal boundary

PAR:

Pseudoautosomal region

PI:

Propidium iodide

RH:

Radiation hybrid

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Acknowledgments

The authors wish to thank Mr. Domenico Incarnato, CNR-ISPAAM, for his excellent technical assistance. Financial support for this study came from the following projects: PSR-Misura 214e2 RARECa, CISIA-VARIGEAV, and by DFG-Grant GO-896/6-3 from the German Research Foundation.

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Authors and Affiliations

  1. Laboratory of Animal Cytogenetics and Gene Mapping, National Research Council of Italy, Institute of Animal Production Systems in Mediterranean Environments (ISPAAM), Naples, Italy

    A. Perucatti, V. Genualdo, A. Iannuzzi & Leopoldo Iannuzzi

  2. Research Unit Molecular Biology, Leibniz Institute for Farm Animal Biology (FBN), Wilhelm-Stahl-Allee 2, 18196, Dummerstorf, Germany

    A. Rebl & T. Goldammer

  3. Department of Soil, Plant, Environment and Animal Production Sciences, University ‘Federico II’ of Naples, Portici, Italy

    D. Di Berardino

  4. CNR-ISPAAM, Via Argine 1085, 80147, Naples, Italy

    Leopoldo Iannuzzi

Authors
  1. A. Perucatti
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  2. V. Genualdo
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  3. A. Iannuzzi
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  4. A. Rebl
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  5. D. Di Berardino
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  6. T. Goldammer
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  7. Leopoldo Iannuzzi
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Corresponding author

Correspondence to T. Goldammer.

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Responsible Editor: Herbert Macgregor.

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Perucatti, A., Genualdo, V., Iannuzzi, A. et al. Advanced comparative cytogenetic analysis of X chromosomes in river buffalo, cattle, sheep, and human. Chromosome Res 20, 413–425 (2012). https://doi.org/10.1007/s10577-012-9285-0

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  • DOI: https://doi.org/10.1007/s10577-012-9285-0

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