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A fast-neutron induced chromosome fragment deletion of 3BS in wheat landrace Wangshuibai increased its susceptibility to Fusarium head blight

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Abstract

Fusarium head blight (FHB), also called wheat scab, is an important disease in warm and humid regions worldwide, which not only reduces crop yield and grain quality, but also is a major safety concern in food and feed production due to mycotoxin contamination. Growing wheat cultivars with FHB resistance is one of the most economical and effective means to control the disease. Chinese wheat landrace Wangshuibai is an important resistant source from southern China. Several resistance QTLs in Wangshuibai were identified and mapped on chromosomes or chromosomal arms including 3BS, 4B, 6BS, 7AL, etc. In the present research, a mutant with increased FHB susceptibility, designated as NAUH117, was identified from the M1 progenies of Wangshuibai irradiated by fast neutron. Genetic analysis of the F1, F2, and F2:3 families from the reciprocal cross of Wangshuibai and NAUH117 indicated that NAUH117 was a recessive mutant. Genome-wide molecular marker analysis identified a deletion in the short arm of chromosome 3B of NAUH117, spanning the region of FL0.57 to FL1.00 that covers the locus of Fhb1 previously mapped on chromosome 3BS. Further molecular cytogenetics characterization by bi-color fluorescence in situ hybridization using three repetitive sequences, pSc119.2, pAs1 and GAA-satellite indicated that a multiple chromosome rearrangements occurred in chromosomes 3B, 6B, 3D, 4D, and 3A of the mutant. During these processes, a distal fragment of chromosome arm 3BS was eliminated, which is confirmed by molecular marker analysis. Four markers covered the deletion fragment were used for analysis of the F2 population. The result showed that the 3BS deletion was only present in the susceptible plants, indicating that the deletion of 3BS fragment in NAUH117 increased susceptibility to FHB. The susceptible mutant will be valuable for the validation of the contribution of the resistant QTL located on 3BS, and for the characterization of the molecular mechanisms of FHB resistance in Wangshuibai.

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Abbreviations

FHB:

Fusarium head blight

QTL:

Quantitative trait locus

FISH:

Fluorescence in situ hybridization

PSS:

Percentage of scabbed spikelets

dpi:

Days post inoculation

GFP:

Green fluorescent protein

PCR:

Polymerase chain reaction

FL:

Fraction length

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Acknowledgments

The research was supported by grants from the National Natural Science Foundation of China (No. 30330380), Programme of Introducing Talents of Discipline to Universities (No. B08025), the Chinese High Tech Program of China (No. 2006AA10Z1F6), the Natural Science Foundation of Jiangsu Province (No. BK2006720), and the McKnight Foundation CCRP Program and Important National Science & Technology Specific Projects of Transgenic Research (No. 2009ZX08002-001B). We are grateful to Dr. Gill (Kansas State University, USA) for useful comments for the research and his critical reading of the manuscript. We thank Dr. Badaeva for her kindly help in the arrangement and the analysis of the FISH results.

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Correspondence to Xiue Wang.

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Responsible Editor: Jiming Jiang.

J. Xiao and X.P. Jia contributed equally to this article.

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Supplemental table 1

Molecular marker on 3BS in identifying of deletion fragment in NAUH117 (DOC 93 kb)

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Xiao, J., Jia, X., Wang, H. et al. A fast-neutron induced chromosome fragment deletion of 3BS in wheat landrace Wangshuibai increased its susceptibility to Fusarium head blight. Chromosome Res 19, 225–234 (2011). https://doi.org/10.1007/s10577-011-9192-9

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  • DOI: https://doi.org/10.1007/s10577-011-9192-9

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