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Studies of male and female meiosis in inv(4)(p1.4;q2.3) pig carriers

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Abstract

Inversions are well-known structural chromosomal rearrangements in humans and pigs. Such rearrangements generally have no effect on the carrier's phenotype. However, the presence of an inversion may impair spermatogenesis and lead to the production of recombinant gametes, responsible for early miscarriages, stillbirth, or congenital abnormalities. This is the first report on meiotic segregation and pairing behavior of the inv(4)(p1.4;q2.3) pericentric inversion in pigs. Despite the very large size of the inverted fragment (76% of the chromosome), SpermFISH results showed that only 4.08% of the gametes produced by male heterozygotes were unbalanced. This low proportion could be explained by the particular behavior of normal and inverted SSC4 chromosomes during the initial stages of meiosis. Indeed, immunohistochemistry analyses revealed that heterosynapsis occurred in 92% of the cells, whereas synaptic adjustment was detected in a few spermatocytes only. Unexpectedly, the proportion of unbalanced gametes produced by female heterozygotes, estimated by FISH on metaphase II oocytes, was also very low (3.69%) and comparable to that in males. According to previous results for male and female meiotic processes, different proportions of recombinant gametes in the two genders would have been expected. Complementary studies should be carried out to further document the meiotic behavior of inversions in pigs.

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Abbreviations

BAC:

Bacterial artificial chromosome

BSA:

Bovine serum albumin

CO:

Crossing-over

DAPI:

4’,6’-Diamidino-2-phenilindole

DNA:

Deoxyribonucleic acid

FISH:

Fluorescence in situ hybridization

FITC:

Fluorescein isothiocyanate

Inv:

Inversion

Mb:

Megabase

MLH1:

MutL Homolog 1

PBS:

Phosphate-buffered saline

SCP3:

Synaptonemal complex protein 3

SSC:

Sus scrofa domestica

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Acknowledgments

The authors would like to thank T. Faraut for constructive discussions on the interest of comparing male and female meiotic segregation patterns in carriers of chromosomal rearrangements.

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Authors and Affiliations

  1. Ecole Nationale Vétérinaire de Toulouse, UMR 444 INRA-ENVT Génétique Cellulaire, 23 chemin des Capelles, BP 87614, 31076, Toulouse Cedex 3, France

    Katia Massip, Martine Yerle, Nathalie Bonnet, Anne Calgaro, Nicolas Mary, Anne-Marie Dudez, Céline Sentenac, Christophe Plard, Alain Ducos & Alain Pinton

  2. Génétique Expérimentale en Productions Animales, INRA Le Magneraud, 17450, Saint Pierre d’Amilly, France

    Yvon Billon

  3. Unité expérimentale Insémination Caprine et Porcine, INRA, 86480, Rouillé, France

    Stéphane Ferchaud

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  1. Katia Massip
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  2. Martine Yerle
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  3. Yvon Billon
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  4. Stéphane Ferchaud
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  5. Nathalie Bonnet
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  6. Anne Calgaro
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  7. Nicolas Mary
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  9. Céline Sentenac
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  10. Christophe Plard
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  11. Alain Ducos
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  12. Alain Pinton
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Correspondence to Alain Pinton.

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Responsible Editor: Walther Traut.

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Massip, K., Yerle, M., Billon, Y. et al. Studies of male and female meiosis in inv(4)(p1.4;q2.3) pig carriers. Chromosome Res 18, 925–938 (2010). https://doi.org/10.1007/s10577-010-9162-7

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  • DOI: https://doi.org/10.1007/s10577-010-9162-7

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