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A- and B-chromosome pairing and recombination in male meiosis of the silver fox (Vulpes vulpes L., 1758, Carnivora, Canidae)

Abstract

We examined A- and B-chromosome pairing and recombination in 12 males from the farm-bred population of the silver fox (2n = 34 + 0–10 Bs) by means of electron and immunofluorescent microscopy. To detect recombination at A and B chromosomes, we used immunolocalisation of MLH1, a mismatch repair protein of mature recombination nodules, at synaptonemal complexes. The mean total number of MLH1 foci at A-autosomes was 29.6 foci per cell. The XY bivalent had one MLH1 focus at the pairing region. Total recombination length of the male fox genome map was estimated as 1,530 centimorgans. We detected single MLH1 foci at 61% of linear synaptic configurations involving B chromosomes. The distribution of the foci along B- and A-bivalents was the same. This may be considered as a first molecular evidence that meiotic recombination does occur in mammalian B chromosomes. There was no correlation between the number of synaptic configurations involving B chromosomes per cell and the recombination rate of the A-genome.

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Abbreviations

ACA:

Anti-centromere antibody

BSA:

Bovine serum albumin

cM:

Centimorgan

DAPI:

4′-6-diamidino-2-phenylindole

Cy3:

Orange fluorescing cyanine

FITC:

Fluorescein isothiocyanate

MLH1:

Homolog of prokaryotic mutL 1 mismatch repair protein

PBS:

Phosphate buffered saline

SC:

Synaptonemal complex

SCP3:

Synaptonemal complex protein 3

S.D.:

Standard deviation

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Acknowledgements

This work was supported by research grants from the Russian Foundation for Basic Research and Russian Ministry of Science and Education, the Programs of Russian Academy of Sciences “Biosphere Origin and Evolution” and “Biodiversity”. We thank Mrs. Marina Rodionova for her help in SC spreading, Prof. Ludmila Trut for valuable discussion and the Microscopic Center of the Siberian Department of the Russian Academy of Sciences for granting access to microscopic equipment.

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Correspondence to Pavel M. Borodin.

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Responsible Editor: Walther Traut.

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Basheva, E.A., Torgasheva, A.A., Sakaeva, G.R. et al. A- and B-chromosome pairing and recombination in male meiosis of the silver fox (Vulpes vulpes L., 1758, Carnivora, Canidae). Chromosome Res 18, 689–696 (2010). https://doi.org/10.1007/s10577-010-9149-4

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Keywords

  • B chromosomes
  • Meiotic recombination
  • Synaptonemal complex
  • MLH1
  • Fox
  • Vulpes vulpes