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Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia)

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Abstract

Three novel families of repetitive DNA sequences were molecularly cloned from the Korean field mouse (Apodemus peninsulae) and characterized by chromosome in-situ hybridization and filter hybridization. They were all localized to the centromeric regions of all autosomes and categorized into major satellite DNA, type I minor, and type II minor repetitive sequences. The type II minor repetitive sequence also hybridized interspersedly in the non-centromeric regions. The major satellite DNA sequence, which consisted of 30 bp elements, was organized in tandem arrays and constituted the majority of centromeric heterochromatin. Three families of repetitive sequences hybridized with B chromosomes in different patterns, suggesting that the B chromosomes of A. peninsulae were derived from A chromosomes and that the three repetitive sequences were amplified independently on each B chromosome. The minor repetitive sequences are present in the genomes of the other seven Apodemus species. In contrast, the major satellite DNA sequences that had a low sequence homology are present only in a few species. These results suggest that the major satellite DNA was amplified with base substitution in A. peninsulae after the divergence of the genus Apodemus from the common ancestor and that the B chromosomes of A. peninsulae might have a species-specific origin.

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Authors and Affiliations

  1. Laboratory of Animal Cytogenetics, Division of Genome Dynamics, Creative Research Initiative ‘Sousei’, Hokkaido University, North 10 West 8, Kita-ku, Sapporo, 060–0810, Japan

    Kazumi Matsubara, Kazuhiko Yamada, Shu Umemoto, Chizuko Nishida & Yoichi Matsuda

  2. Chromosome Science Labo Inc., Shiroishi-ku, Sapporo, 003–0801, Japan

    Kazuhiko Yamada

  3. Laboratory of Wild Animals, Department of Zootechnical Sciences, Faculty of Agriculture, Tokyo University of Agriculture, Kanagawa, 234–0034, Japan

    Kimiyuki Tsuchiya

  4. Applied Biology Co. Ltd, Minato-ku,, Tokyo, 107–0062, Japan

    Kimiyuki Tsuchiya

  5. Department of Applied Life Science, Faculty of Bioscience and Biotechnology, Sojo University, Kumamoto, 860–0082, Japan

    Naoki Ikeda & Takahito Chijiwa

  6. Laboratory of Animal Cytogenetics, Division of Life Science, Graduate School of Life Science, Hokkaido University, Sapporo, 060–0810, Japan

    Chizuko Nishida & Yoichi Matsuda

  7. RIKEN BioResource Center, Tsukuba, 305–0074, Japan

    Kazuo Moriwaki

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  1. Kazumi Matsubara
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  2. Kazuhiko Yamada
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  4. Kimiyuki Tsuchiya
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  9. Yoichi Matsuda
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Correspondence to Kazumi Matsubara.

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Matsubara, K., Yamada, K., Umemoto, S. et al. Molecular cloning and characterization of the repetitive DNA sequences that comprise the constitutive heterochromatin of the A and B chromosomes of the Korean field mouse (Apodemus peninsulae, Muridae, Rodentia). Chromosome Res 16, 1013–1026 (2008). https://doi.org/10.1007/s10577-008-1259-x

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  • DOI: https://doi.org/10.1007/s10577-008-1259-x

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