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Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus

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Abstract

Two highly repeated DNAs, designated NmE1/NmE2 and NmE5, were identified by EcoRV digestion in the chiton Nuttallochiton mirandus (Mollusca: Polyplacophora). The comparison of the sequences obtained showed high similarity in 5′ and 3′ regions and the NmE5 sequence displayed an inserted sequence that might arise from a transposable element. Southern blotting analyses suggested a tandem organization of both satellite DNA families identified. Moreover, dot blot analyses, performed on several molluscan species, revealed a different degree of conservation of the repeated DNAs. Fluorescence in-situ hybridizations (FISH) on metaphase chromosomes showed that both satellite DNAs are located at centromeric regions.

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Authors and Affiliations

  1. Istituto di Biologia e Genetica, Facoltà di Scienze, Università Politecnica delle Marche, via Brecce Bianche, I-60131, Ancona, Italy

    Maria Assunta Biscotti, Marco Barucca, Ettore Olmo & Adriana Canapa

  2. Dipartimento di Biologia Evolutiva e Comparata, Università di Napoli ‘Federico II’, via Mezzocannone 8, Napoli, Italy

    Teresa Capriglione

  3. Dipartimento di Biologia Strutturale e Funzionale, Complesso Monte S. Angelo, Università di Napoli ‘Federico II’, via Cinthia, Napoli, Italy

    Gaetano Odierna

Authors
  1. Maria Assunta Biscotti
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  2. Marco Barucca
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  3. Teresa Capriglione
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  4. Gaetano Odierna
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  5. Ettore Olmo
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  6. Adriana Canapa
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Correspondence to Adriana Canapa.

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Biscotti, M.A., Barucca, M., Capriglione, T. et al. Molecular and cytogenetic characterization of repetitive DNA in the Antarctic polyplacophoran Nuttallochiton mirandus . Chromosome Res 16, 907–916 (2008). https://doi.org/10.1007/s10577-008-1248-0

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  • DOI: https://doi.org/10.1007/s10577-008-1248-0

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