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Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker—the largest subtelocentric chromosome pair as revealed by cross-species painting

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Abstract

Leuciscine cyprinids possess a nearly invariant diploid number (2n = 50) with an extremely uniform karyotype comprising of 8 pairs of metacentric, 13–15 pairs of submetacentric and 2–4 pairs of subtelocentric (st) to acrocentric (a) chromosomes. The largest pair is characteristically an st/a element—the ‘leuciscine’ cytotaxonomic marker. Previously, the interspecific homology of this chromosome pair could not be assessed owing to the inability to produce euchromatic or serial banding patterns. In the present study, we used laser-microdissection (15–20 copies of the marker chromosome) to construct a whole chromosome probe (WCP) from the marker chromosome of the roach Rutilus rutilus to ascertain the interspecific homology of marker chromosomes by cross-species in-situ hybridization. WCP was hybridized to chromosomes of widely distributed (Abramis brama, Alburnoides bipunctatus, Alburnus alburnus, Aspius aspius, Ballerus ballerus, B. sapa, Blicca bjoerkna, Chondrostoma nasus, Leucaspius delineatus, Leuciscus leuciscus, L. idus, R. rutilus, Scardinius erythrophthalmus, Squalius cephalus, and Vimba vimba) and Iberian endemic species (Achondrostoma oligolepis, Iberochondrostoma almacai, I. lusitanicum, Pseudochondrostoma duriense, S. alburnoides and S. pyrenaicus). Cross-species in-situ hybridization to chromosomes of Phoxinus phoxinus, a representative of leuciscine sister lineage, showed the same pattern as in all of the leuciscins. The probe consistently hybridized to the distal part of the short arm of the marker chromosome, indicating sequence homology.

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Authors and Affiliations

  1. Laboratory of Fish Genetics, Institute of Animal Physiology and Genetics, Czech Academy of Sciences, 277 21, Liběchov, Czech Republic

    Petr Ráb, Marie Rábová & Šárka Pelikánová

  2. Faculdade de Ciências, Departamento de Biologia Animal/Centro de Biologia Ambiental, Universidade de Lisboa, Campo Grande C2 – Piso 3, 1749-016, Lisbon, Portugal

    Carla Sofia Pereira & Maria João Collares-Pereira

  3. Joint Laboratory of Genetics, Physiology and Reproduction of Fish, Institute of Animal Physiology and Genetics, Academy of Sciences of the Czech Republic and Research Institute of Fish Culture and Hydrobiology, University of South Bohemia České Budějovice, 389 25, Vodňany, Czech Republic

    Petr Ráb

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  1. Petr Ráb
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  2. Marie Rábová
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  4. Maria João Collares-Pereira
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  5. Šárka Pelikánová
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Correspondence to Petr Ráb.

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Ráb, P., Rábová, M., Pereira, C.S. et al. Chromosome studies of European cyprinid fishes: interspecific homology of leuciscine cytotaxonomic marker—the largest subtelocentric chromosome pair as revealed by cross-species painting. Chromosome Res 16, 863–873 (2008). https://doi.org/10.1007/s10577-008-1245-3

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  • DOI: https://doi.org/10.1007/s10577-008-1245-3

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