Abstract
Nine bacterial artificial chromosomes containing genes linked to the Z chromosome of the zebra finch (Taeniopygia guttata) were localized using FISH on synaptonemal complex spreads. Their positions were correlated with those previously reported on the mitotic Z chromosome, showing a linear relationship between positions along the mitotic chromosome and its synaptonemal complex. Distances in cM between the genes were calculated using a cytological map of the crossing-over based on the distribution of MLH1 foci along the ZZ synaptonemal complex (MLH1-cM map). It is shown that physical and genetic distances lack a linear relationship along most of the chromosome length, due to clustering of crossover events around the telomeres. This relationship departs strongly from that observed in the chicken Z chromosome and reflects the existence of different recombination rates and patterns among birds in spite of wide genomic conservation.
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References
Anderson LK, Doyle GG, Brigham B, et al. (2003) High-resolution crossover maps for each bivalent of Zea mays using recombination nodules. Genetics 165: 849–865.
Anderson LK, Salameh N, Bass HW, et al. (2004) Integrating genetic linkage maps with pachytene chromosome structure in maize. Genetics 166: 1923–1933.
Backström N, Qvarnstrom A, Gustafsson L, Ellegren H (2006) Levels of linkage disequilibrium in a wild bird population. Biol Lett 2: 435–438.
Backström N, Fagerberg S, Ellegren H (2008) Genomics of natural bird populations: a gene-based set of reference markers evenly spread across the avian genome. Mol Ecol 17: 964–980.
Baker SM, Plug AW, Prolla TA, et al. (1996) Involvement of mouse Mlh1 in DNA mismatch repair and meiotic crossing over. Nat Genet 13: 336–342.
Calderón PL, Pigozzi MI (2006) MLH1-focus mapping in birds shows equal recombination between sexes and diversity of crossover patterns. Chromosome Res 14: 605–612.
Carpenter AT (1975) Electron microscopy of meiosis in Drosophila melanogaster females: II. The recombination nodule—a recombination-associated structure at pachytene? Proc Natl Acad Sci U S A 72: 3186–3189.
Chang SB, Anderson LK, Sherman JD, Royer SM, Stack SM (2007) Predicting and testing physical locations of genetically mapped loci on tomato pachytene chromosome 1. Genetics 176: 2131–2138.
Dawson DA, Akesson M, Burke T, Pemberton JM, Slate J, Hansson B (2007) Gene order and recombination rate in homologous chromosome regions of the chicken and a passerine bird. Mol Biol Evol 24: 1537–1552.
Derjusheva S, Kurganova A, Habermann F, Gaginskaya E (2004) High chromosome conservation detected by comparative chromosome painting in chicken, pigeon and passerine birds. Chromosome Res 12: 715–723.
Ellegren H (2005) The avian genome uncovered. Trends Ecol Evol 20: 180–186.
Froenicke L, Anderson LK, Wienberg J, Ashley T (2002) Male mouse recombination maps for each autosome identified by chromosome painting. Am J Hum Genet 71: 1353–1368.
Gregory TR, Nicol JA, Tamm H, et al. (2007) Eukaryotic genome size databases. Nucleic Acids Res 35: 332–338.
Hernandez-Hernandez A, Rincon-Arano H, et al. (2008) Differential distribution and association of repeat DNA sequences in the lateral element of the synaptonemal complex in rat spermatocytes. Chromosoma 117: 77–87.
Itoh Y, Arnold AP (2005) Chromosomal polymorphism and comparative painting analysis in the zebra finch. Chromosome Res 13: 47–56.
Itoh Y, Kampf K, Arnold AP (2006) Comparison of the chicken and zebra finch Z chromosomes shows evolutionary rearrangements. Chromosome Res 14: 805–815.
Lawrence CJ, Seigfried TE, Bass HW, Anderson LK (2006) Predicting chromosomal locations of genetically mapped loci in maize using the Morgan2McClintock Translator. Genetics 172: 2007–2009.
Marcon E, Moens P (2003) MLH1p and MLH3p Localize to precociously induced chiasmata of okadaic-acid-treated mouse spermatocytes. Genetics 165: 2283–2287.
Moens PB, Kolas NK, Tarsounas M, Marcon E, Cohen PE, Spyropoulos B (2002) The time course and chromosomal localization of recombination-related proteins at meiosis in the mouse are compatible with models that can resolve the early DNA-DNA interactions without reciprocal recombination. J Cell Sci 115: 1611–1622.
Organ CL, Shedlock AM, Meade A, Pagel M, Edwards SV (2007) Origin of avian genome size and structure in non-avian dinosaurs. Nature 446: 180–184.
Peters AH, Plug AW, van Vugt MJ, de Boer P (1997) A drying-down technique for the spreading of mammalian meiocytes from the male and female germline. Chromosome Res 5: 66–68.
Peterson DG, Lapitan NL, Stack SM (1999) Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Genetics 152: 427–439.
Pigozzi MI (2001) Distribution of MLH1 foci on the synaptonemal complexes of chicken oocytes. Cytogenet Cell Genet 95: 129–133.
Pigozzi MI (2007) Localization of single-copy sequences on chicken synaptonemal complex spreads using fluorescence in situ hybridization (FISH). Cytogenet Genome Res 119: 105–112.
Pigozzi MI, Solari AJ (1998) Germ cell restriction and regular transmission of an accessory chromosome that mimics a sex body in the zebra finch, Taeniopygia guttata. Chromosome Res 6: 105–113.
Pigozzi MI, Solari AJ (1999a) Recombination nodule mapping and chiasma distribution in spermatocytes of the pigeon, Columba livia. Genome 42: 308–314.
Pigozzi MI, Solari AJ (1999b) Equal frequencies of recombination nodules in both sexes of the pigeon suggest a basic difference with eutherian mammals. Genome 42: 315–321.
Pigozzi MI, Solari AJ (2005) The germ-line-restricted chromosome in the zebra finch: recombination in females and elimination in males. Chromosoma 114: 403–409.
Rahn MI, Solari AJ (1986) Recombination nodules in the oocytes of the chicken, Gallus domesticus. Cytogenet Cell Genet 43: 187–193.
Reeves A (2001) MicroMeasure: a new computer program for the collection and analysis of cytogenetic data. Genome 44: 439–443.
Schmid M, Nanda I, Hoehn H, et al. (2005) Second report on chicken genes and chromosomes 2005. Cytogenet Genome Res 109: 415–479.
Sherman JD, Stack SM (1995) Two-dimensional spreads of synaptonemal complexes from solanaceous plants. VI. High-resolution recombination nodule map for tomato (Lycopersicon esculentum). Genetics 141: 683–708.
Solari AJ (1980) Synaptosomal complexes and associated structures in microspread human spermatocytes. Chromosoma 81: 315–337.
Stack SM (1984) Heterochromatin, the synaptonemal complex and crossing over. J Cell Sci 71: 159–176.
Stapley J, Birkhead TR, Burke T, Slate J (2008) A linkage map of the zebra finch Taeniopygia guttata provides new insights into avian genome evolution. Genetics 179: 651–667.
Sun F, Oliver-Bonet M, Liehr T, et al. (2004) Human male recombination maps for individual chromosomes. Am J Hum Genet 74: 521–531.
Wahlberg P, Stromstedt L, Tordoir X, et al. (2007) A high-resolution linkage map for the Z chromosome in chicken reveals hot spots for recombination. Cytogenet Genome Res 117: 22–29.
Zickler D, Kleckner N (1999) Meiotic chromosomes: integrating structure and function. Annu Rev Genet 33: 603–754.
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Pigozzi, M.I. Relationship between physical and genetic distances along the zebra finch Z chromosome. Chromosome Res 16, 839–849 (2008). https://doi.org/10.1007/s10577-008-1243-5
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DOI: https://doi.org/10.1007/s10577-008-1243-5