Advertisement

Chromosome Research

, Volume 14, Issue 7, pp 767–776 | Cite as

Triploid origin of the gibel carp as revealed by 5S rDNA localization and chromosome painting

  • H. P. Zhu
  • D. M. Ma
  • J. F. Gui
Article

Abstract

5S ribosomal DNA (rDNA) was isolated and sequenced from the gibel carp Carassius auratus gibelio with 162 chromosomes and crucian carp Carassius auratus with 100 chromosomes, and fluorescent probes for chromosome localization were prepared to ascertain the ploidy origin and evolutionary relationship between the two species. Using fluorescence in-situ hybridization (FISH), major 5S rDNA signals were localized to the short arms of three subtelocentric chromosomes in the gibel carp and to the short arms of two subtelocentrics in the crucian carp. In addition, some minor signals were detected on other chromosomes of both species. Simultaneously, six chromosomes were microdissected from the gibel carp metaphase spreads using glass needles, and the isolated chromosomes were amplified in vitro by degenerate oligonucleotide primed-polymerase chain reaction (DOP-PCR). Significantly, when the DOP-PCR-generated probes prepared from each single chromosome were hybridized, three same-sized chromosomes were painted in each gibel carp metaphase, whereas only two painted chromosomes were observed in each crucian carp metaphase spread. The data indicate that gibel carp is of triploid origin in comparison with diploid crucian carp.

Key words

Carassius auratus gibelio chromosome painting 5S rDNA fluorescence in-situ hybridization gynogenesis microdissection triploidy 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Affonso PR, Pedro MG (2005) Chromosomal diversification of reef fishes from genus Centropyge (Perciformes, Pomacanthidae). Genetica 123: 227–233.PubMedCrossRefGoogle Scholar
  2. Chen H, Yang J, Xue GX, Zhu LH (1994) The application of RAPD in allogynogenetic silver crucian carp (Carassius auratus gibelio). Chinese Sci Bull 39: 661–664.Google Scholar
  3. Cherfas NB (1981) Gynogenesis in fishes. In Kirpichnikov VS, ed. Genetic Bases of Fish Selection. Berlin: Springer-Verlag, pp. 255–273.Google Scholar
  4. Dong CH, Yang ST, Yang ZA, Zhang L, Gui JF (2004) A C-type lectin associated and translocated with cortical granules during oocyte maturation and egg fertilization in fish. Dev Biol 265: 341–354.PubMedCrossRefGoogle Scholar
  5. Fan Z, Shen J (1990) Studies on the evolution of bisexual reproduction in crucian carp (Carassius auratus gibelio Bloch). Aquaculture 84: 235–244.CrossRefGoogle Scholar
  6. Gui JF (1989) Evolutionary genetics of unisexual vertebrates. Nat J (Shanghai) 12: 116–122.Google Scholar
  7. Gui JF (1996) A unique study system: gynogenetic fish Carassius auratus gibelio. Sci Found China 4: 44–46.Google Scholar
  8. Gui, JF (1999) Fish developmental genetics and artificial propagation. In Wu C, Gui JF, eds., Fish Genetics and Breeding Engineering. Shanghai: Shanghai Scientific and Technical Publishers, pp. 41–62.Google Scholar
  9. Gui JF, Liang SC, Sun JM, Huang JM, Jiang YG (1990) Studies on genome manipulation in fish, I. Induction of triploid transparent colored crucian carp (Carassius auratus transparent colored variety) by hydrostatic pressure. Acta Hydrobiol Sinica 14: 336–344.Google Scholar
  10. Gui JF, Zhu LF, Wei XH et al. (1997) Genetic diversity and breeding implications of gynogenetic silver crucian carp. Hereditas (Beijing) 19: 37–38.Google Scholar
  11. Huang CJ, Huang FL, Wang YC, Hsiao PW, Lo TB (1993) Molecular cloning and relationship of highly repetitive HindIII sequences in three cyprinid species: silver carp, bighead carp and grass carp. Proc Natl Sci Counc Repub China Part B Life Sci 17: 85–90.Google Scholar
  12. Jiang YG, Yu HX, Chen BD, Liang SC (1983) Biological effect of heterologous sperm on gynogenetic offspring in Carassius auratus gibelio. Acta Hydrobiol Sinica 8: 1–13.Google Scholar
  13. Kobayashi H, Nakano K, Nakamura M (1977) On the hybrids, 4n ginbuna (C. auratus langsdorfii) ×kinbuna (C. auratus subsp.) and their chromosome. Bull Jpn Soc Sci Fish 43: 31–37.Google Scholar
  14. Long EO, David ID (1980) Repeated genes in eucaryotes. Annu Rev Biochem 49: 727–764.PubMedCrossRefGoogle Scholar
  15. Ma XF, Gustafson JP (2005) Genome evolution of allopolyploids: a process of cytological and genetic diploidization. Cytogenet Genome Res 109: 236–249.PubMedCrossRefGoogle Scholar
  16. Martins CP, Galetti PM (2001) Organization of 5S rDNA in species of the fish Leporinus: two different genomic locations are characterized by distinct nontranscribed spacers. Genome 44: 903–910.PubMedCrossRefGoogle Scholar
  17. Murakami M, Fujitani H (1998) Characterization of repetitive DNA sequences carrying 5S rDNA of the triploid ginbuna (Japanese silver crucian carp, Carassius auratus langsdorfi). Genes Genet Syst 73: 9–20.PubMedCrossRefGoogle Scholar
  18. Ohno S, Muramoto J, Christian L (1967) Diploid–tetraploid relationship among old-world members of fish family Cyprinidae. Chromosoma 23: 1–9.CrossRefGoogle Scholar
  19. Reid T, Schröck E, Ning Y, Wineberg J (1998) Chromosome painting: a useful art. Hum Mol Genet 7: 1619–1626.CrossRefGoogle Scholar
  20. Sambrook J, Fritsch EF, Maniatis T (1989) Molecular Cloning: A Laboratory Manual, 2nd edn. New York: Cold Spring Harbor Laboratory, pp. 463–468.Google Scholar
  21. Schartl M, Nanda I, Schlupp I et al. (1995) Incorporation of subgenomic amounts of hostspecies DNA in the gynogenetic Amazon Molly. Nature 373: 68–71.CrossRefGoogle Scholar
  22. Telenius H, Pelmear AH, Tunnacliffe A et al. (1992) Cytogenetic analysis by chromosome painting using DOP-PCR amplified flow-sorted chromosomes. Gene Chrom Cancer 4: 257–263.Google Scholar
  23. Venkatesh B (2003) Evolution and diversity of fish genomes. Curr Opin Genet Dev 13: 588–592.PubMedCrossRefGoogle Scholar
  24. Vrijenhoek RC (1994) Unisexual fish: model system for study ecology and evolution. Annu Rev Ecol Syst 25: 71–96.CrossRefGoogle Scholar
  25. Wasko AP, Martins C, Wright JM, Galetti PM (2001) Molecular organization of 5S rDNA in fishes of the genus Brycon. Genome 44: 893–902.PubMedCrossRefGoogle Scholar
  26. Wei WH, Zhang J, Zhang YB, Zhou L, Gui JF (2003) Genetic heterogeneity and ploidy level analysis among different gynogenetic clones of polyploid gibel carp. Cytometry 56: 46–52.PubMedCrossRefGoogle Scholar
  27. Yang L, Gui JF (2004) Positive selection on multiple antique allelic lineages of transferrin in the polyploid Carassius auratus. Mol Biol Evol 21: 1264–1277.PubMedCrossRefGoogle Scholar
  28. Yi MS, Li YQ, Liu JD, Zhou L, Yu QX, Gui JF (2003) Molecular cytogenetic detection of paternal chromosome fragments in allogynogenetic gibel carp, Carassius auratus gibelio Bloch. Chrom Res 11: 665–671.PubMedCrossRefGoogle Scholar
  29. Yogeeswaran K, Frary A, York TL et al. (2005) Comparative genome analyses of Arabidopsis spp.: inferring chromosomal rearrangement events in the evolutionary history of A. thaliana. Genome Res 15: 505–515.PubMedCrossRefGoogle Scholar
  30. Yu XJ, Zhou T, Li YC, Li K, Zhou M (1989) Chromosomes of Chinese Fresh-water Fishes. Beijing: Science Press, pp. 75–77.Google Scholar
  31. Zhou L, Gui JF (2002) Karyotypic diversity in polyploid gibel carp, Carassius auratus gibelio Bloch. Genetica 115: 223–232.PubMedCrossRefGoogle Scholar
  32. Zhou L, Wang Y, Gui JF (2000) Genetic evidence for gonochoristic reproduction in gynogenetic silver crucian carp (Carassius auratus gibelio) as revealed by RAPD assays. J Mol Evol 51: 498–506.PubMedGoogle Scholar

Copyright information

© Springer 2006

Authors and Affiliations

  1. 1.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology, Chinese Academy of SciencesGraduate School of the Chinese Academy of SciencesWuhanChina

Personalised recommendations