Abstract
An image enhancement procedure was developed to produce high-contrast chromosome paint images. This procedure is well suited for images where brightness–contrast enhancement is subjective. Three examples are given to show that the procedure is very efficient to remove non-specific hybridization signals from the chromosome paint image. Chromosomes of roe deer contain large amounts of centromeric heterochromatic DNA. Echidna chromosomes show specific heterochromatic DNA distributed over several chromosomes. In both cases chromosome identification was hampered by bright heterochromatic regions. The enhancement tool was fully used in cross-species chromosome painting, which is the last example. The three examples show that the procedure is very simple to use and removes background in a controlled and defined manner.
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Murphy WJ, Larkin DM, Everts-van der Wind A et al. (2005) Dynamics of mammalian chromosome evolution inferred from multispecies comparative maps. Science 309(5734): 613–617.
Rens W, O’Brien PCM, Yang F, Graves JAM, Ferguson-Smith MA (1999) Karyotype relationships between four distantly related marsupials revealed by reciprocal chromosome painting. Chromosome Res 7: 461–474.
Rens W, Grützner F, O’Brien PC, Fairclough H, Graves JA, Ferguson-Smith MA (2004) Resolution and evolution of the duck-billed platypus karyotype with an X1Y1X2Y2X3Y3X4Y4X5Y5 male sex chromosome constitution. Proc Natl Acad Sci USA 16;101(46): 16257–16261.
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Rens, W., Moderegger, K., Skelton, H. et al. A procedure for image enhancement in chromosome painting. Chromosome Res 14, 497–503 (2006). https://doi.org/10.1007/s10577-006-1056-3
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DOI: https://doi.org/10.1007/s10577-006-1056-3