Abstract
Lanthanum (La) is a natural rare-earth element that can damage the central nervous system and impair learning and memory. However, its neurotoxic mechanism remains unclear. In this study, adult female rats were divided into 4 groups and given distilled water solution containing 0%, 0.125%, 0.25%, and 0.5% LaCl3, respectively, and this was done from conception to the end of the location. Their offspring rats were used to establish animal models to investigate LaCl3 neurotoxicity. Primary neurons cultured in vitro were treated with LaCl3 and infected with LKB1 overexpression lentivirus. The results showed that LaCl3 exposure resulted in abnormal axons in the hippocampus and primary cultured neurons. LaCl3 reduced the expression of LKB1, p-LKB1, STRAD and MO25 proteins, and directly or indirectly affected the expression of LKB1, leading to decreased activity of LKB1-MARK2 and LKB1-STK25-GM130 pathways. This study indicated that LaCl3 exposure could interfere with the normal effects of LKB1 in the brain and downregulate LKB1-MARK2 and LKB1-STK25-GM130 signaling pathways, resulting in abnormal axon in offspring rats.
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Enquiries about data availability should be directed to the authors.
Change history
21 June 2022
A Correction to this paper has been published: https://doi.org/10.1007/s10571-022-01244-1
Abbreviations
- Ace:
-
Acetylated α-tubulin
- Dil:
-
1,1'-Dioctadecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate
- GM130:
-
Golgi Matrix Protein130
- LaCl3 :
-
Lanthanum chloride
- LKB1:
-
Liver kinase B1
- MARK2:
-
Microtubule affinity-regulating kinases 2
- MO25:
-
Mouse protein 25
- NC:
-
Negative control
- NSE:
-
Neuron-specific enolase
- OE:
-
Over expression
- REEs:
-
Rare-earth elements
- STE20:
-
Sterile twenty
- STK25:
-
Serine/threonine kinase 25
- STRAD:
-
STE20-related adapter
- Tyr:
-
Tyrosinated α-tubulin
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This study was supported by the National Natural Science Foundation of China (No.81773469).
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JY was responsible for the experimental design and revised the paper. ZS performed the experiments and wrote the manuscript. HM, JL and WS created the models. SW, XL and CJ provided technical support.
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Song, Z., Mao, H., Liu, J. et al. Lanthanum Chloride Induces Axon Abnormality Through LKB1-MARK2 and LKB1-STK25-GM130 Signaling Pathways. Cell Mol Neurobiol 43, 1181–1196 (2023). https://doi.org/10.1007/s10571-022-01237-0
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DOI: https://doi.org/10.1007/s10571-022-01237-0