Abstract
miRNA therapy is popularly investigated in treating acute spinal cord injury (SCI) and offers a significant prospect for the treatment of acute SCI. We aimed to provide pre-clinical validations of miRNA in the treatment of SCI. A systematic search of EMBASE, PubMed, Web of Science, the Cochrane Library, and Scopus databases was performed. Rats, which were the most used animals (70%, n = 46 articles), receiving miRNA therapy got prominent recovery in SCI models [BBB score, SMD 3.90, 95% CI 3.08–4.73, p < 0.01]. Locomotor function of fore and hind limbs in SCI mice receiving miRNA therapy (30%, n = 19 articles) [grip strength, SMD 3.22, 95% CI 2.14–4.26; p < 0.01; BBB score, SMD 3.47, 95% CI 2.38–4.56, p < 0.01; BMS, SMD 2.27, 95% CI 1.34–3.20, p < 0.01] also recovered better than mice in control group. Then, we conducted the subgroup analysis and did find that high-quality articles trended to report non-therapeutic effect of miRNA. Furtherly, we analyzed 46 miRNAs, including 9 miRNA families (miR-21-5p/34a-3p/124-3p/126-3p/223-3p/543-3p/30-3p/136-3p/15-5p), among which miR-30-3p/136-3p/15-5p family were not effective in recovering locomotor function of rats. Conclusively, miRNAs are curative drugs for SCI, however, appropriate miRNA carrier and which miRNA is the most efficacious for SCI should be furtherly investigated.
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YCS and YW: Conceptualization, YW and HXY: methodology, YW and HXY: investigation, YW: software, YW and HXY: formal analysis, YW and YCS: writing—original draft, YCS: writing—review & editing, YW and YCS: supervision. All authors read and approved the final manuscript.
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Supplementary file5 (PDF 614 KB) Additional file 5. TSAs of the effect of exosomes on locomotor recovery after SCI. A The adjusted required information size is based on a median value of mean BBB scores of 3.90, an overall significance level (α) of 0.05, a type II risk (β) of 0.1 (power 90%), and equals 423 rats (vertical dotted red line). The cumulative Z-curve (solid blue line) connected by individual studies (small squares) crosses the upper O'Brien–Fleming monitoring boundary of benefit (descending dotted red line). B The adjusted required information size is based on the least value of mean BBB scores of 1, an overall significance level (α) of 0.05, a type II risk (β) of 0.1 (power 90%), and equals 2117 rats (vertical dotted red line). The cumulative Z-curve (solid blue line) connected by individual studies (small squares) crosses the upper O'Brien–Fleming monitoring boundary of benefit (descending dotted red line)
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Supplementary file8 (PDF 902 KB) Additional file 8. Funnel plot for the strength of pair limbs (A), left fore limbs (B) and right fore limbs (C)
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Supplementary file10 (PDF 622 KB) Additional file 10. Funnel plot for BBB score of mice at the moment 35th day post-injury
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Wang, Y., Yi, H. & Song, Y. miRNA Therapy in Laboratory Models of Acute Spinal Cord Injury in Rodents: A Meta-analysis. Cell Mol Neurobiol 43, 1147–1161 (2023). https://doi.org/10.1007/s10571-022-01235-2
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DOI: https://doi.org/10.1007/s10571-022-01235-2