Abstract
Cav1.2 channel phosphorylation plays an important role in regulating neuronal plasticity by action potential-dependent Ca2+ entry. Most studies of Cav1.2 regulation by phosphorylation have been reported in heart and muscles. Here, we identified phosphorylation sites of neuronal Cav1.2 channel protein purified from rat brain using mass spectrometry. The functional characterization of these phosphorylation sites showed altered voltage-dependent biophysical properties of the channel, without affecting current density. These results show that neuronal Cav1.2 channel is regulated by phosphorylation in a complex mechanism involving multiple phosphorylation sites.
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Acknowledgements
The authors are grateful to Dr. D. Lipscombe for gifts of plasmids (Cav1.2, Cavβ3, and Cavα2δ1).
Funding
This work was supported by grants from the National Research Foundation of Korea (NRF) funded by Korean government (MIST) (NRF-2019R1A2C2003642 to K.S.P.) and the Basic Science Research Program (2018R1D1A1B07043142 to J.H.L.).
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K.S.P. conceived the project. S.K.S., H.Y.L., and K.C. performed the experiments. S.K.S., H.Y.L., K.C., Y.W.C., J.S.Y., J.H.L., and K.S.P. analyzed and interpreted the data. S.K.S. and K.S.P. wrote the paper with input from the other authors. The authors read and approved the final manuscript.
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All animal experiments were performed in compliance with guidelines approved by the Institutional Animal Care and Use Committee (IACUC) of Kyung Hee University.
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Informed consent was obtained from all individual participants included in this study.
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Shin, S.K., Li, H.Y., Cho, K. et al. Sites and Regulation of L-Type Ca2+ Channel Cav1.2 Phosphorylation in Brain. Cell Mol Neurobiol 42, 2427–2431 (2022). https://doi.org/10.1007/s10571-021-01095-2
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DOI: https://doi.org/10.1007/s10571-021-01095-2