Excitatory neurotransmission relies on the precise targeting of α-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA)-type glutamate receptors to the neuronal plasma membrane. Activity-dependent ubiquitination of AMPA receptor (AMPAR) subunits sorts internalised receptors to late endosomes for degradation, which ultimately determines the number of AMPARs on neuronal membrane. Our recent study has demonstrated a functional cross-talk between the phosphorylation and ubiquitination of the GluA1 subunit in mammalian central neurons. However, the existence of such a cross modulation for the GluA2 subunit remains unknown. Here, we have shown that bicuculline induced GluA2 ubiquitination on the same lysine residues (Lys-870 and Lys-882) in the C-terminal as those elicited by the AMPA treatment. Interestingly, bicuculline-induced ubiquitination was markedly enhanced by the phospho-mimetic GluA2 S880E mutant. Pharmacological activation of protein kinase C (PKC) by phorbol ester, which mediates the phosphorylation of GluA2 at Ser-880, augmented bicuculline-induced ubiquitination of GluA2 in cultured neurons. This effect was specific for the GluA2 subunit because phorbol ester did not alter the level of GluA1 ubiquitination. However, phorbol ester-induced enhancement of GluA2 ubiquitination did not require Ser-880 phosphorylation. This suggests that pseudo-phosphorylation of Ser-880 is sufficient but is not necessary for the augmentation of bicuculline-induced GluA2 ubiquitination. Collectively, these data provide the first demonstration of subunit-specific modulation of AMPAR ubiquitination by the PKC-dependent signalling pathway in mammalian central neurons.
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Days in vitro
Glutamate receptor-interacting protein 1
L-type voltage-gated calcium channels
Metabotropic glutamate receptors
Postsynaptic density 95/disc large/zona occludens-1
Protein interacting with C-kinase 1
Protein kinase A
Protein kinase C
Phorbol 12-myristate 13-acetate
Ring finger protein
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This work was supported in part by grants from the Australian National Health and Medical Research Council (GNT1138452), the Alzheimer’s Australia Dementia Research Foundation (DPG14-57), and the Clem Jones Centre for Ageing Dementia Research (to VA). JW was supported by an Australian Research Council DECRA Fellowship (DE170100112). SG was a recipient of University of Queensland International Scholarship.
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All authors declare that they have no competing financial interests.
All animal handling procedures were carried out in accordance to the Australian code for the care and use of animals for scientific purposes by the National Health and Medical Research Council and were approved by the University of Queensland Animal Ethics Committee (AEC approval number QBI/047/18).
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Widagdo, J., Kerk, J.W., Guntupalli, S. et al. Subunit-Specific Augmentation of AMPA Receptor Ubiquitination by Phorbol Ester. Cell Mol Neurobiol 40, 1213–1222 (2020). https://doi.org/10.1007/s10571-020-00809-2
- AMPA receptors
- Phorbol ester
- Protein kinase C