Photobiomodulation Therapy Inhibit the Activation and Secretory of Astrocytes by Altering Macrophage Polarization

Sun, Jiakai; Zhang, Jiawei; Li, Kun; Zheng, Qiao; Song, Jiwei; Liang, Zhuowen; Ding, Tan; Qiao, Lin; Zhang, Jianxin; Hu, Xueyu; Wang, Zhe

doi:10.1007/s10571-019-00728-x

Original Research
Published: 24 August 2019

Photobiomodulation Therapy Inhibit the Activation and Secretory of Astrocytes by Altering Macrophage Polarization

Jiakai Sun¹^na1,
Jiawei Zhang¹^na1,
Kun Li¹^na1,
Qiao Zheng¹,
Jiwei Song¹,
Zhuowen Liang¹,
Tan Ding¹,
Lin Qiao²,
Jianxin Zhang³,
Xueyu Hu¹ &
Zhe Wang¹

Cellular and Molecular Neurobiology volume 40, pages 141–152 (2020)Cite this article

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Abstract

Spinal cord injury (SCI) stimulates reactive astrogliosis and the infiltration of macrophages, which interact with each other at the injured area. We previously found Photobiomodulation (PBM) significantly decreases the number of M1 macrophages at the injured area of SCI. But the exact nature of the astrocyte response following PBM and relationship with the macrophage have not been explored in detail. In this study, a BALB/c mice model with standardized bilateral spinal cord compression and a macrophage-astrocyte co-culture model were applied to study effects of PBM on astrocytes. Results showed that PBM inhibit the expression of the astrocyte markers glial fibrillary acidic protein (GFAP) and the secretion of chondroitin sulfate proteoglycans (CSPG) in the para-epicenter area, decrease the number of M1 macrophage in vivo. The in vitro experiments indicated M1 macrophages promote the cell viability of astrocytes and the expression of CSPG. However, PBM significantly inhibited the expression of GFAP, decreased activation of astrocyte, and downregulated the expression of CSPG by regulating M1 macrophages. These results demonstrate that PBM may regulate the interaction between macrophages and astrocytes after spinal cord injury, which inhibited the formation of glial scar.

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Authors Contributions

ZW and XYH designed and supervised experiments; JKS, JWZ, KL,TD, XYH and ZW wrote the manuscript and analyzed the data; JKS, JWZ, KL, QZ, ZWL and LQ developed the PBM-Macrophage irradiation model and performed the molecular biology experiment; JKS, JWZ, KL, QZ, JWS, JXZ performed the immunofluorescence staining.

Funding

This study was funded by Grants from the National Natural Science Foundation of China (Grant Nos. 81572151, 81070996, 81101352) and Science and Technology Department of Shaanxi Province (Grant Nos. 2016SF-143, 2017SF-021).

Author information

Jiakai Sun, Jiawei Zhang, Kun Li contributed equally to this work.

Affiliations

Department of Orthopedics, Xijing Hospital, Fourth Military Medical University, Changle West Road No. 127, Xi’an, 710032, Shaanxi, China
Jiakai Sun, Jiawei Zhang, Kun Li, Qiao Zheng, Jiwei Song, Zhuowen Liang, Tan Ding, Xueyu Hu & Zhe Wang
Department of Orthopedics, Third Hospital of Chinese PLA, Baoji, Shaanxi, China
Lin Qiao
Department of Orthopedics, Weinan Central Hospital, Weinan, Shaanxi, China
Jianxin Zhang

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Jiakai Sun
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Jiawei Zhang
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Kun Li
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Qiao Zheng
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Jiwei Song
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Zhuowen Liang
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Tan Ding
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Lin Qiao
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Jianxin Zhang
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Xueyu Hu
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Zhe Wang
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Correspondence to Xueyu Hu or Zhe Wang.

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Jiakai Sun, Jiawei Zhang, Kun Li, Qiao Zheng, Jiwei Song, Zhuowen Liang, Tan Ding, Lin Qiao, Jianxin Zhang, Xueyu Hu, Zhe Wang declare that they have no conflicts of interest. The authors alone are responsible for the content and writing of the paper.

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All animal care and experimental programs were conducted according to standard All applicable international and national ethical guidelines (National Institutes of Health Guide to the use of Laboratory Animals) and approved by the Animal Care and Use Committee of the Fourth Military Medical University. All efforts were made to minimize the number of mice used and their suffering.

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Sun, J., Zhang, J., Li, K. et al. Photobiomodulation Therapy Inhibit the Activation and Secretory of Astrocytes by Altering Macrophage Polarization. Cell Mol Neurobiol 40, 141–152 (2020). https://doi.org/10.1007/s10571-019-00728-x

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Received: 06 May 2019
Accepted: 14 August 2019
Published: 24 August 2019
Issue Date: January 2020
DOI: https://doi.org/10.1007/s10571-019-00728-x

Keywords

Spinal cord injury
Photobiomodulation
Astrocyte
Macrophage
Chondroitin sulfate proteoglycans