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Cellular and Molecular Neurobiology

, Volume 38, Issue 4, pp 891–899 | Cite as

Ameliorating Effect of Osteopontin on H2O2-Induced Apoptosis of Human Oligodendrocyte Progenitor Cells

  • Neda Mazaheri
  • Maryam Peymani
  • Hamid Galehdari
  • Kamran Ghaedi
  • Ali Ghoochani
  • Abbas Kiani-Esfahani
  • Mohammad Hossein Nasr-Esfahani
Original Research
  • 141 Downloads

Abstract

Recently our group used oligodendrocyte progenitor cells (OPCs) as appropriate model cells to pinpoint the mechanism of the progress of neurodegenerative disorders. In the present study, we focused on the therapeutic role of osteopontin (OPN), a secreted glycosylated phosphoprotein, involved in a number of physiological events including bone formation and remodeling, immune responses, and tumor progression. Protective role of OPN, as a negative regulator of tumorigenesis, has already been clarified. Human embryonic stem cell-derived OPCs were pretreated with OPN before induction of apoptosis by H2O2. Data indicated that OPN prohibited cell death and enhanced OPC viability. This effect is achieved through reduction of apoptosis and induction of anti-apoptosis markers. In addition OPN induces expression of several integrin subunits, responsible for OPN interaction. Notably, our findings showed that expression of αV β1/β3/β5 and β8 integrins increased in response to OPN, while treatment with H2O2 down-regulated αV β1/β5 and β8 integrins expression significantly. In conclusion, OPN may act via αV integrin signaling and trigger suppression of P53-dependent apoptotic cascades. Therefore OPN therapy may be considered as a feasible process to prevent progress of neurodegenerative diseases in human.

Keywords

OPC OPN H2O2 Apoptosis 

Abbreviations

ANOVA

One-way analysis of variance

bFGF

Basic fibroblast growth factor

CNS

Central nervous system

EBs

Embryoid bodies

ECM

Extracellular matrix

EGF

Epidermal growth factor

FITC

Fluorescein isothiocyanate

hESC

Human embryonic stem cell

Lam

Laminin

MBP

Myelin basic protein

MS

Multiple sclerosis

OLs

Oligodendrocytes

OPCs

Oligodendrocyte progenitor cells

OPN

Osteopontin

PBS

Phosphate-buffered saline

PDGF

Platelet-derived growth factor

PLL

Poly-l-laminin

RA

Retinoic acid

RGD

Arg–Gly–Asp

ROS

Reactive oxygen species

RT-qPCR

Real-time quantitative PCR

SEM

Standard error of mean

TRITC

Tetramethyl rhodamine isothiocyanate

Notes

Acknowledgements

We thank our colleagues for their association and helpful discussions in this study.

Author Contributions

NM was involved in concept and design, acquisition, analysis and interpretation of data, and drafting of the manuscript. MP was involved in supervision, and contributed to the design of work, analysis, and interpretation of data, and drafted sections of manuscript. HG was involved in supervision and contributed to design of work. KG was involved in supervision, and contributed to the design of work, analysis, and interpretation of data, critical revision of the manuscript for important intellectual content, and finalized the manuscript. AG contributed to design the work. AKE was involved in acquisition of data and drafted sections of manuscript. MHNE was involved in supervision, and contributed to the design of work, administrative, technical, or material support.

Compliance with Ethical Standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Science+Business Media, LLC 2017

Authors and Affiliations

  • Neda Mazaheri
    • 1
  • Maryam Peymani
    • 2
    • 4
  • Hamid Galehdari
    • 1
  • Kamran Ghaedi
    • 3
    • 4
  • Ali Ghoochani
    • 4
  • Abbas Kiani-Esfahani
    • 4
  • Mohammad Hossein Nasr-Esfahani
    • 3
    • 4
  1. 1.Department of Genetics, Faculty of ScienceShahid Chamran University of AhvazAhvazIran
  2. 2.Department of Biology, Faculty of Basic Sciences, Shahrekord BranchIslamic Azad UniversityShahrekordIran
  3. 3.Department of Biology, Faculty of SciencesUniversity of IsfahanIsfahanIran
  4. 4.Department of Cellular Biotechnology, Cell Science Research CenterRoyan Institute for Biotechnology, ACECRIsfahanIran

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