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Characterization of the Expression of Basigin Gene Products Within the Pineal Gland of Mice

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Abstract

The expression of Basigin gene products and monocarboxylate transporter-1 (MCT1) has been investigated within the mammalian neural retina and suggests a role for these proteins in cellular metabolism within that tissue. The purpose of the present study was to investigate the expression of these same proteins in the pineal gland of the mouse brain. Mouse pineal gland and neural retina RNA and protein were subjected to quantitative reverse transcription-polymerase chain reaction and immunoblotting analyses. In addition, paraffin-embedded sections of each tissue were analyzed for expression of Basigin gene products and MCT1 via immunohistochemistry. The results indicate that MCT1 and Basigin variant-2, but not Basigin variant-1, are expressed within the mouse pineal gland. The expression of Basigin variant-2 and MCT1 was localized to the capsule surrounding the gland. The position and relative amounts of the gene products suggest that they play a much less prominent role within the pineal gland than in the neural retina.

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Acknowledgements

This work was supported by NIH F32EY13918, the University of North Florida Offices of Academic Affairs and Undergraduate Studies (to JDO), and by NSF IBN-0113697 (to PJL).

Author’s Contribution

D.T. performed the q-RT-PCR, immunoblotting, and immunohistochemical analyses; L.v E. and P.L. generated the paraffin-embedded samples for immunohistochemistry; Judith Ochrietor generated the confocal micrographs.

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Authors and Affiliations

  1. Department of Biology, University of North Florida, 1 UNF Drive, Jacksonville, FL, 32224, USA

    Derek Tokar & Judith D. Ochrietor

  2. Whitney Laboratory for Marine Biosciences and the Department of Anatomy and Cell Biology, University of Florida, St. Augustine, FL, USA

    Leslie van Ekeris & Paul J. Linser

Authors
  1. Derek Tokar
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  2. Leslie van Ekeris
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  3. Paul J. Linser
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  4. Judith D. Ochrietor
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Corresponding author

Correspondence to Judith D. Ochrietor.

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All procedures performed in studies involving animals were in accordance with the ethical standards of the University of Florida and the University of North Florida. All applicable guidelines for the care and use of animals were followed.

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Tokar, D., van Ekeris, L., Linser, P.J. et al. Characterization of the Expression of Basigin Gene Products Within the Pineal Gland of Mice. Cell Mol Neurobiol 37, 1141–1145 (2017). https://doi.org/10.1007/s10571-016-0441-5

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  • DOI: https://doi.org/10.1007/s10571-016-0441-5

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