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Wogonin Inhibits LPS-Induced Inflammatory Responses in Rat Dorsal Root Ganglion Neurons Via Inhibiting TLR4–MyD88–TAK1-Mediated NF-κB and MAPK Signaling Pathway

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Abstract

Recent studies showed that the activation of toll-like receptor 4 (TLR4) on dorsal root ganglion (DRG) neurons might underlie neuropathic and inflammatory pain states. This study was undertaken to investigate the effects of wogonin, a flavonoid with potent anti-inflammatory properties on the inflammatory reaction and TLR4 dependent pathways in lipopolysaccharide (LPS)-treated DRG neurons. Our results showed that wogonin not only inhibited the expression and interaction of TLR4, MyD88, and TAK1, but also reduced the activation of nuclear factor kappa B and mitogen-activated protein kinases pathway in LPS-treated DRG neurons. Moreover, wogonin significantly suppressed the release of pro-inflammatory mediators in LPS-induced DRG neurons, including cyclooxygenase-2, inducible nitric oxide synthases, interleukin (IL)-1β, IL-6, and tumor necrosis factor-alpha. Our results suggested that pre-treatment with wogonin could attenuate the TLR4-mediated inflammatory response in LPS-induced DRG neurons, thus might be beneficial for the treatment of neuropathic and inflammatory pain.

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Authors and Affiliations

  1. Department of Anesthesiology, The First Affiliated Hospital of Nanchang University, Nanchang, Jiangxi, People’s Republic of China

    Shibiao Chen, Jiangqin Xiong, Yanping Zhan, Weicheng Liu & Xiuhong Wang

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  1. Shibiao Chen
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  2. Jiangqin Xiong
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  3. Yanping Zhan
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  4. Weicheng Liu
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  5. Xiuhong Wang
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Correspondence to Shibiao Chen.

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Chen, S., Xiong, J., Zhan, Y. et al. Wogonin Inhibits LPS-Induced Inflammatory Responses in Rat Dorsal Root Ganglion Neurons Via Inhibiting TLR4–MyD88–TAK1-Mediated NF-κB and MAPK Signaling Pathway. Cell Mol Neurobiol 35, 523–531 (2015). https://doi.org/10.1007/s10571-014-0148-4

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