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MicroRNA-29c/PTEN Pathway is Involved in Mice Brain Development and Modulates Neurite Outgrowth in PC12 Cells

Cellular and Molecular Neurobiology volume 35, pages 313–322 (2015)Cite this article

An Erratum to this article was published on 04 May 2017

Abstract

Growing evidence indicates that microRNAs (miRNAs) are important mediators of brain development and neurite growth. However, the affected signaling mechanisms are not clearly clarified. In the present study, we confirm that miR-29c is expressed during mice brain development and increases neurite outgrowth via decreasing PTEN expression. We first screen the picked-out miR-29c up-regulated in PC12 cells induced by nerve growth factor (NGF). In silico analysis of possible miR-29c targets, VEGFA, MAPK3, PDGFB, and PTEN mRNA are proposed as relatively likely putative binding sites for miR-29c. Subsequently, we detect that miR-29c is involved in brain development and has a negative relationship with the expression of PTEN. Then, using luciferase reporter assay,we demonstrate that miR-29c could directly target to the 3′-UTR of PTEN mRNA and result in down-expression of PTEN. By infecting PC12 cells with lentiviral pLKO-miR-29c or control, we also find that increasing levels of miR-29c markedly increase Akt phosphorylation level, and thus, promote neurite outgrowth of PC12 cells. Together, our results identify that miR-29c is required for mice brain development and modulates neurite outgrowth in PC12 cells via targeting PTEN and has a promising therapeutic target for neural disease.

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Acknowledgments

This study was supported by Grants from the Changzhou Science & Technology Bureau (CJ20122027) and the National Science Foundation of China (8137218, 81471263).

Conflict of interest

We state that the manuscript, or parts of it, have not been and will not be submitted elsewhere for publication. Hongjun Zou, Ya Ding, Weifeng Shi, Xu Xu, Aihua Gong, Zhijian Zhang, Jinbo Liu have agreed to the submission of the final manuscript and declare no conflicts of interest.

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Affiliations

  1. Department of Orthopedics, the Third Affiliated Hospital of Suzhou University, No. 185 Juqian street, Changzhou, Jiangsu, 213003, China

    Hongjun Zou, Ya Ding, Xu Xu & Jinbo Liu

  2. Department of Clinical Laboratory, the Third Affiliated Hospital of Suzhou University, No. 185 Juqian Street, Changzhou, Jiangsu, 213003, China

    Weifeng Shi

  3. School of Medicine, Jiangsu University, Zhenjiang, 212013, China

    Aihua Gong & Zhijian Zhang

  4. Department of Orthopaedics, The First People’s Hospital of Changzhou, School of Medicine, Third Affiliated Hospital of Suzhou University, No. 185 of Juqian Street, Changzhou, 213000, China

    Jinbo Liu

Authors
  1. Hongjun Zou
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  2. Ya Ding
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  3. Weifeng Shi
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  4. Xu Xu
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  5. Aihua Gong
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  6. Zhijian Zhang
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  7. Jinbo Liu
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Corresponding author

Correspondence to Jinbo Liu.

Additional information

An erratum to this article is available at http://dx.doi.org/10.1007/s10571-017-0493-1.

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Zou, H., Ding, Y., Shi, W. et al. MicroRNA-29c/PTEN Pathway is Involved in Mice Brain Development and Modulates Neurite Outgrowth in PC12 Cells. Cell Mol Neurobiol 35, 313–322 (2015). https://doi.org/10.1007/s10571-014-0126-x

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  • DOI: https://doi.org/10.1007/s10571-014-0126-x

Keywords

  • miRNA
  • Brain development
  • Neurite outgrowth
  • miR-29c
  • PTEN
  • Akt