Cellular and Molecular Neurobiology

, Volume 34, Issue 7, pp 999–1010 | Cite as

Immunoregulation Effects of Bone Marrow-Derived Mesenchymal Stem Cells in Xenogeneic Acellular Nerve Grafts Transplant

  • Lihong Fan
  • Zefeng Yu
  • Jia Li
  • Xiaoqian Dang
  • Kunzheng Wang
Original Research


This study evaluated whether bone marrow-derived mesenchymal stem cells (BM-MSCs) combined with xenogeneic acellular nerve grafts (xANGs) would reduce the inflammation reaction of xANGs transplantation. BM-MSCs were extracted, separated, purified, and cultured from the bone marrow of rats. Then BM-MSCs were seeded into 5 mm xANGs as experimental group, while xANGs group was chosen as control. Subcutaneous implantation and nerve grafts transplantation were done in this study. Walking-track tests, electrophysiological tests, H&E staining, and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations, cytokine concentrations of IL-2, IL-10, IFN-γ and TNF-α in lymphocytes supernatants and serum of the two groups were evaluated. Walking-track tests and electrophysiological tests suggested the group of BM-MSCs with xANGs obtained better results than xANGs group (P < 0.05). H&E staining and immunostaining of CD4, CD8, and CD68 of subcutaneous implantations showed there were less inflammatory cells in the group of BM-MSCs when compared with the xANGs group. The cytokine concentrations of IL-2, IFN-γ, and TNF-α in BM-MSCs group were lower than xANGs group in lymphocytes supernatants and serum (P < 0.05). However, IL-10 concentrations in BM-MSCs group were higher than xANGs group (P < 0.05). xANG with BM-MSCs showed better nerve repair function when compared with xANG group. Furthermore, xANG with BM-MSCs showed less inflammatory reaction which might indicate the reason of its better nerve regeneration.


Immunoregulation Xenogeneic acellular nerve graft Bone marrow-derived mesenchymal stem cells Transplant 



We would like to thank the National Natural Science Foundation of China (Grant Nos. 81101363 and 81371944) for financially supporting this study.

Conflict of interest

The authors declare that they have no conflict of interest.


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Copyright information

© Springer Science+Business Media New York 2014

Authors and Affiliations

  • Lihong Fan
    • 1
  • Zefeng Yu
    • 1
  • Jia Li
    • 1
  • Xiaoqian Dang
    • 1
  • Kunzheng Wang
    • 1
  1. 1.The First Department of OrthopedicsThe Second Affiliated Hospital of Xi’an Jiaotong UniversityXi’anChina

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