Skip to main content

Advertisement

Log in

Increased Expression of TBP/TFIID After Spinal Cord Injury in Adult Rats

  • Original Research
  • Published:
Cellular and Molecular Neurobiology Aims and scope Submit manuscript

Abstract

Transcription factor IID (TFIID), as a general transcription factor, plays a pivotal role in the preinitiation complex (PIC) assembly and transcription initiation by recruiting RNA polymerase II to the promoter. The TFIID complex contains the TATA-box binding protein (TBP) and a group of conserved TAF proteins. However, its distribution and function in the central nervous system (CNS) are more diverse than previously understood. Here, we mainly investigated the spatiotemporal expression and cellular localization of TBP/TFIID during spinal cord injury (SCI) in adult rats. Western blot analysis revealed that TBP/TFIID was present in normal rat’s spinal cord. It gradually increased, reached a peak at the third day after SCI, and then decreased. We observed that TBP/TFIID was widely distributed in spinal cord, mainly in neurons and glial cells. In addition, Western blot detection also showed that the third day post-injury was the proliferation peak indicated by the elevated expression of proliferating cell nuclear antigen (PCNA), a marker of proliferating cells. Importantly, injury-induced expression of TBP/TFIID was colabelled by PCNA showed the increase of TBP/TFIID expression in proliferating astrocytes and microglia. Collectively, we hypothesize that TBP/TFIID may be implicated in the proliferation of astrocytes and microglia and the recovery of neurological outcomes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Fig. 1
Fig. 2
Fig. 3
Fig. 4

Similar content being viewed by others

References

  • Andel F III, Ladurner AG et al (1999) Three-dimensional structure of the human TFIID-IIA-IIB complex. Science 286(5447):2153–2156

    Article  CAS  PubMed  Google Scholar 

  • Burley SK, Roeder RG (1996) Biochemistry and structural biology of transcription factor IID (TFIID). Annu Rev Biochem 65:769–799

    Article  CAS  PubMed  Google Scholar 

  • Byrnes KR, Stoica BA et al (2007) Cell cycle activation contributes to post-mitotic cell death and secondary damage after spinal cord injury. Brain: A Journal of Neurology 130(Pt 11):2977–2992

    Article  Google Scholar 

  • Chen XH, Iwata A et al (2003) Neurogenesis and glial proliferation persist for at least one year in the subventricular zone following brain trauma in rats. J Neurotrauma 20(7):623–631

    Article  PubMed  Google Scholar 

  • D’Alessio JA, Wright KJ et al (2009) Shifting players and paradigms in cell-specific transcription. Mol Cell 36(6):924–931

    Article  PubMed Central  PubMed  Google Scholar 

  • Davidson I (2003) The genetics of TBP and TBP-related factors. Trends Biochem Sci 28(7):391–398

    Article  CAS  PubMed  Google Scholar 

  • Di Giovanni S, Knoblach SM et al (2003) Gene profiling in spinal cord injury shows role of cell cycle in neuronal death. Ann Neurol 53(4):454–468

    Article  PubMed  Google Scholar 

  • Dumont RJ, Okonkwo DO, Verma S, Hurlbert RJ, Boulos PT, Ellegala DB et al (2001) Acute spinal cord injury, part I: pathophysiologic mechanisms. Clin Neuropharmacol 24:254–264

    Article  CAS  PubMed  Google Scholar 

  • Friedman MJ, Shah AG et al (2007) Polyglutamine domain modulates the TBP-TFIIB interaction: implications for its normal function and neurodegeneration. Nat Neurosci 10(12):1519–1528

    Article  CAS  PubMed  Google Scholar 

  • Goodrich JA, Tjian R (2010) Unexpected roles for core promoter recognition factors in cell-type-specific transcription and gene regulation. Nat Rev Genet 11(8):549–558

    CAS  PubMed Central  PubMed  Google Scholar 

  • Greenblatt J (1991) Roles of TFIID in transcriptional initiation by RNA polymerase II. Cell 66(6):1067–1070

    Article  CAS  PubMed  Google Scholar 

  • Gruner JA (1992) A monitored contusion model of spinal cord injury in the rat. J Neurotrauma 9(2):123–126 discussion 126–128

    Article  CAS  PubMed  Google Scholar 

  • Hampsey M (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62:465–503

    CAS  PubMed Central  PubMed  Google Scholar 

  • Hochheimer A, Zhou S et al (2002) TRF2 associates with DREF and directs promoter-selective gene expression in Drosophila. Nature 420(6914):439–445

    Article  CAS  PubMed  Google Scholar 

  • Kwon BK, Tetzlaff W, Grauer JN et al (2004) Pathophysiology and pharmacologic treatment of acute spinal cord injury. Spine J 4:451–464

    Article  PubMed  Google Scholar 

  • Liu Z, Wang D et al (2011) Increased expression of transcription initiation factor IIB after rat traumatic brain injury. J Mol Histol 42(3):265–271

    Article  CAS  PubMed  Google Scholar 

  • McDonald JW, Sadowsky C (2002) Spinal-cord injury. Lancet 359:417–425

    Article  PubMed  Google Scholar 

  • McGraw J, Hiebert GW, Steeves JD (2001) Modulating astrogliosis after neurotrauma. J Neurosci Res 63(2):109–115

    Article  CAS  PubMed  Google Scholar 

  • Morganti-Kossmann MC, Rancan M et al (2002) Inflammatory response in acute traumatic brain injury: a double-edged sword. Curr Opin Crit Care 8(2):101–105

    Article  PubMed  Google Scholar 

  • Mrak RE, Griffin WS (2005) Glia and their cytokines in progression of neurodegeneration. Neurobiol Aging 26:349–354

    Article  CAS  PubMed  Google Scholar 

  • Myer VE, Young RA (1998) RNA polymerase II holoenzymes and subcomplexes. J Biol Chem 273(43):27757–27760

    Article  CAS  PubMed  Google Scholar 

  • Orphanides G, Lagrange T et al (1996) The general transcription factors of RNA polymerase II. Genes Dev 10(21):2657–2683

    Article  CAS  PubMed  Google Scholar 

  • Shen A, Liu Y, Zhao J, Qin J, Shi S, Chen M, Gao S, Xiao F, Lu Q, Cheng C (2008) Temporal-spatial expressions of p27kip1 and its phosphorylation on Serine-10 after acute spinal cord injury in adult rat: implications for post-traumatic glial proliferation. Neurochem Int 52(6):1266–1275

    Article  CAS  PubMed  Google Scholar 

  • Somera-Molina KC, Robin B, Somera CA et al (2007) Glial activation links early-life seizures and long-term neurologic dysfunction: evidence using a small molecule inhibitor of proinflammatory cytokine upregulation. Epilepsia 48:1785–1800

    Article  CAS  PubMed  Google Scholar 

  • Tatarakis A, Margaritis T et al (2008) Dominant and redundant functions of TFIID involved in the regulation of hepatic genes. Mol Cell 31(4):531–543

    Article  CAS  PubMed  Google Scholar 

  • Tator CH (1995) Update on the pathophysiology and pathology of acute spinal cord injury. Brain Pathol 5(4):407–413

    Article  CAS  PubMed  Google Scholar 

  • Tora L (2002) A unified nomenclature for TATA box binding protein (TBP)-associated factors (TAFs) involved in RNA polymerase II transcription. Genes Dev 16(6):673–675

    Article  CAS  PubMed  Google Scholar 

  • Van Dyke MW, Sawadogo M (1990) DNA-binding and transcriptional properties of human transcription factor TFIID after mild proteolysis. Mol Cell Biol 10(7):3415–3420

    PubMed Central  PubMed  Google Scholar 

  • Wei H, Teng H et al (2012) An upregulation of SENP3 after spinal cord injury: implications for neuronal apoptosis. Neurochem Res 37(12):2758–2766

    Article  CAS  PubMed  Google Scholar 

  • Yang J, Cao J et al (2013) Transcription initiation factor IIb involves in schwann cell differentiation after rat sciatic nerve crush. J Mol Neurosci 49(3):491–498

    Article  CAS  PubMed  Google Scholar 

  • Yu X, Wen H et al (2013) Temporal and spatial expression of KIF3B after acute spinal cord injury in adult rats. J Mol Neurosci 49(2):387–394

    Article  CAS  PubMed  Google Scholar 

  • Zhang J, Li D et al (2013) Expression of RBMX after spinal cord injury in rats. J Mol Neurosci 49(2):417–429

    Article  CAS  PubMed  Google Scholar 

  • Zhao J, Zhang S et al (2011) KPC1 expression and essential role after acute spinal cord injury in adult rat. Neurochem Res 36(3):549–558

    Article  CAS  PubMed  Google Scholar 

Download references

Conflict of interest

All authors declare that there are no conflicts of interest.

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to Jian Zhao.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ding, T., Wen, H., Wei, H. et al. Increased Expression of TBP/TFIID After Spinal Cord Injury in Adult Rats. Cell Mol Neurobiol 34, 669–677 (2014). https://doi.org/10.1007/s10571-014-0048-7

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s10571-014-0048-7

Keywords

Navigation